Establishment of pure Dendrobium nobile variety similarity judging method, pure Dendrobium nobile variety similarity judging standard and pure Dendrobium nobile variety similarity judging method

Abstract

The invention relates to an establishment of a pure Dendrobium nobile variety similarity judging method, a pure Dendrobium nobile variety similarity judging standard and a pure Dendrobium nobile variety similarity judging method. The establishment process of the method comprises the following steps: S1, collecting samples of Dendrobium nobile consistent with the gene sequencing conclusion, and measuring the second internode stem diameter of each sample; S2, performing normality test on a variable of the second internode stem diameter of each sample; and S3, standard interval: if the results obtained in the step S2 obey the normal distribution, obtaining 95% confidence interval of a mean and 95% confidence interval of a standard deviation according to the normal distribution population calculation formula, wherein the confidence intervals can be used as a standard range for identifying the purity of unknown samples. The judging standard established according to the collected data is asfollows: 95% confidence interval of a mean and 95% confidence interval of a standard deviation are (8.3677, 9.3422) and (2.4923, 3.1872), respectively, and the tested Dendrobium nobile samples only need to be compared with the intervals. The invention can identify the purity of the variety through the related morphological characteristics of Dendrobium nobile, so that the value or the existence ofartificially planted Dendrobium nobile can be simply judged. The accuracy of identification is high, which has important practical significance.

Description

technical field [0001] The present invention relates to the fields of medicine and biology, and in particular to the establishment of a method for judging the similarity of Dendrobium nobile purebred, the judgment standard and the judgment method, that is, the method for establishing the correlation between the morphological characteristics of Dendrobium nobile and the conclusion of gene sequencing The identification method for measuring the purity of Dendrobium nobile species, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. The genus Dendrobium is the largest genus in the Orchidaceae plant, including Dendrobium glabra, Dendrobium fringe, Dendrobium officinale, Dendrobium multiflora, Dendrobium dendrobii, and Dendrobium nobile. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history of medicinal use. It was liste...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium nobile

However, the long-term artificial planting has also brought about false ones and substandard ones for Dendrobium nobile. This is because ① artificial planting has changed the growth environment of wild Dendrobium nobile; , the application of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium nobile; ③ and because there are many varieties of Dendrobium, the hybridization between its varieties makes the traits of its close relatives cross; ④ other uncontrollable Or unmeasurable factors, in summary, the medicinal ingredients of some artificially planted Dendrobium nobile have changed, and even important medicinal ingredients have disappeared. The gene sequence is also substantially different from the original wild gene sequence

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