Establishment process of judgment method for purebred similarity of dendrobium officinale, judgment standard and judgment method

Abstract

The invention relates to an establishment process of a judgment method for purebred similarity of dendrobium officinale, a judgment standard and the judgment method. The establishment process of the judgment method comprises the steps of S1, collecting dendrobium officinale samples consistent with a gene sequencing conclusion, and measuring the stem diameter of each sample; S2, carrying out normality test on a stem diameter variable of each sample; and S3, obtaining standard intervals: if a result obtained in the step S2 obeys normal distribution, obtaining a 95% confidence interval of a meanvalue and a 95% confidence interval of a standard deviation according to an overall calculation formula of the normal distribution, wherein the confidence intervals can serve as standard ranges for identifying the purity of unknown samples. The judgment standard established according to the actually collected data is that the 95% confidence interval of the mean value and the 95% confidence interval of the standard deviation are (5.8079, 6.3227) and (0.9256, 1.2954) respectively, and the tested dendrobium officinale samples only need to be compared with the intervals. According to the method disclosed by the invention, the purity of the variety of the dendrobium officinale can be identified through the morphology-related characteristics of the dendrobium officinale, so that the value of theartificially planted dendrobium officinale or whether the value of the artificially planted dendrobium officinale is existent or not can be simply judged; and the method is high in identification accuracy, and has important practical significance.

Description

technical field [0001] The present invention relates to the fields of medicine and biology, in particular to the establishment of a method for judging the similarity of Dendrobium candidum pure species, the judgment standard and the judgment method, that is, the method for establishing the correlation between the morphological characteristics of Dendrobium candidum and the conclusion of gene sequencing and the method to be tested. The identification method of the purity of Dendrobium officinale species, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium officinale, Dendrobium fringe, Dendrobium officinale, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and many other varieties. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history ...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium candidum.

However, long-term artificial planting has also brought false ones to false ones and shoddy good ones. This is because ① artificial planting has changed the growth environment of wild Dendrobium candidum; The spread of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium candidum; ③And because there are many varieties of Dendrobium, the hybridization between the varieties makes the phenomenon of trait crossover among its close relatives; ④Others are uncontrollable or unmeasurable In summary, the medicinal ingredients of some artificially planted Dendrobium officinale have changed, and even important medicinal ingredients have disappeared. There are substantial differences in the wild gene sequence

Once the medicinal value of these Dendrobium candidum weakens or disappears, and the field continues to use it without knowing it, the consequences will be very serious. more serious consequences

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