Vertical type culture dish and system and method thereof
A petri dish and vertical technology, applied in the field of vertical petri dish and its system, can solve the problems of inability to transport the petri dish, cumbersome operation, increase of medium infection, etc., to reduce the number of perfusion medium, improve sealing, The effect of avoiding infection
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Embodiment 1
[0030] Such as figure 1 As shown, the vertical culture dish of the present invention comprises a lower dish cover 1, a upper dish cover 4 and a dish plate 6, and the dish plate 6 is placed between the lower dish cover 1 and the upper dish cover 4, and the lower dish cover 1 outside The wall is provided with two openings I2 through the upper and lower petri dishes, the opening I2 is arranged in a cross shape, and the end of the opening I2 is provided with a sealing member 3; the periphery of the dish upper cover 4 is provided with an elastic fastening device 5, The upper cover 4 of the dish is closely attached to the lower cover 1 of the dish through the fastening device 5 .
[0031] The core of the first embodiment is that two symmetrically arranged openings I2 are provided on the outer wall of the lower cover 1 of the dish. The function of the opening I2 is to connect the upper and lower vertical culture dishes, so as to facilitate the perfusion of medium into multiple verti...
Embodiment 2
[0044] The difference between this embodiment 2 and embodiment 1 is that although two passages are also used, the lower cover 1 of the dish has only one opening I2, while the other passage uses the lower cover 1, the upper cover 4 and the dish. 6 channels instead.
[0045] Such as Figure 5 As shown, the vertical culture dish of the present invention comprises a lower dish cover 1, a upper dish cover 4 and a dish plate 6, and the dish plate 6 is placed between the lower dish cover 1 and the upper dish cover 4, and the lower dish cover 1 outside The wall is provided with at least one opening I2 through the upper and lower petri dishes, the opening I2 is arranged in a cross shape, and the end of the opening I2 is provided with a sealing member 3; the periphery of the dish upper cover 4 is provided with an elastic fastening device 5, The upper cover 4 of the dish is closely attached to the lower cover 1 of the dish through the fastening device 5 .
[0046] Such as Figure 6 an...
Embodiment 3
[0059] In addition to the vertical perfusion method mentioned in Embodiment 1 and Embodiment 2, the method for using the vertical culture dish of the present invention can also adopt a horizontal perfusion method.
[0060] The present invention adds up through the mutual connection of the openings on both sides of the lower cover 1 of the dish. When pouring the medium into the vertical culture dish, it can start from the opening I2 on one side of the vertical culture dish, or start from the opening I2 on the other side of the vertical culture dish. Before pouring the medium, add the vertical culture The dishes can be placed in stacks or horizontally. Wait for the medium to fill the vertical culture dish system and let it stand for a period of time. After the medium solidifies, separate the vertical culture dish and seal the opening.
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