A kind of detection method of ofloxacin residue in livestock and poultry hair
A technology of ofloxacin and a detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of easy deterioration of ofloxacin detection samples, inconvenient sampling, and difficulty in storage, etc., so as to shorten the preparation time and save The effect of pretreatment time and easy operation
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[0049] 1. Preparation of standard solution
[0050] Preparation of standard solution: Accurately weigh an appropriate amount of standard products of ofloxacin and ofloxacin-D3, and dissolve them in methanol to prepare a 100.0-1000.0 mg / L standard solution.
[0051] Preparation of standard stock solution: Pipette an appropriate amount of standard solution into a 10.0mL volumetric flask, dilute to the mark with methanol, and prepare a 1.0mg / L standard stock solution.
[0052] Preparation of standard working solution: properly dilute the standard stock solution with methanol-water solution (1:1).
[0053] 2. Linear regression equation and detection limit
[0054]Prepare a series of ofloxacin standard working solutions with a concentration of 0.05 μg / L-100.0 μg / L. The internal standard ofloxacin-D3 concentration is 50.0 μg / L, and the peak area ratio (y) is used for the content (x). Figure, the result shows that, in the scope of 0.05 μ g / L-100.0 μ g / L, peak area ratio (y) and con...
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