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RFF2 cell construction method

A construction method, RFF2 technology, applied in animal cells, vertebrate cells, blood/immune system cells, etc., can solve the problem that immune cells cannot balance attack and defense, and achieve self-protection ability, improve adaptability, and high safety. Effect

Inactive Publication Date: 2019-01-04
BEIJING DCTY BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the combination of polypeptide impact, in vitro expansion of cells, and in vitro sealing, a kind of T cell with specific killing effect and integrated attack and defense is provided, which solves the problem that immune cells cannot balance attack and defense in cellular immunotherapy, with good precision and high killing rate

Method used

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Embodiment Construction

[0044] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0045] Such as figure 1 As shown, the embodiment of the present invention provides a method for constructing RFF2 cells, which is used to construct a specific T cell for cellular immunotherapy, named RFF2 cells. The method comprises the steps of:

[0046] S1) PBMC cells (peripheral blood mononuclear cells, peripheral blood mononuclear cells) are loaded with tumor-causing mutation polypeptides, and then the PBMC cells loaded with polypeptides are...

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Abstract

The invention discloses and provides an RFF2 cell construction method, and belongs to the field of biotechnology. The invention discloses the method used for constructing an offensive and defensive specific T cell having the specific lethal effect, and the specific T cell is an RFF2 cell used for the cellular immunotherapy, and the accuracy is high and the lethality is high. The construction method comprises the following steps: enabling a PBMC (Peripheral Blood Mononuclear Cell) to load a polypeptide causing the tumor mutation, impacting the polypeptide of the PBMC loaded with the polypeptide; amplifying culturing after the impacting to obtain an FF cell; using the polypeptide causing the tumor mutation as an antigen to directly simulate the FF cell to screen the accurate polypeptide; using the accurate polypeptide to load the PBMC cell, then culturing, using the accurate polypeptide to impact for multiple times in the culturing process; continuing to culture after the impacting, obtaining an RFF cell; closing an immunosuppressive signal molecule of the RFF cell in vitro by using an antibody like PD1, and obtaining the RFF2 cell. The construction method can be used in the cellularimmunotherapy technique.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing RFF2 cells. Background technique [0002] Tumor cell immunotherapy is an emerging tumor treatment model. It collects immune cells from the patient, then cultures and expands them in vitro, and then infuses them back into the patient to stimulate and enhance the body's autoimmune function to treat tumors. Tumor cell immunotherapy is the fourth tumor treatment method after surgery, radiotherapy and chemotherapy. [0003] Normal or bioengineered human cells are transplanted or infused into patients, and the newly infused cells can replace damaged cells, or have stronger immune killing functions, so as to achieve the purpose of treating diseases. [0004] The bioengineered cells are modified by special methods in the in vitro culture process, which can improve their lethality and effectively kill tumor cells in patients. For example, Chinese patent application...

Claims

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Application Information

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IPC IPC(8): C12N5/0783C12N1/38
CPCC12N1/38C12N5/0638
Inventor 焦顺昌张嵘周子珊解佳森王海燕李营营
Owner BEIJING DCTY BIOTECH CO LTD
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