Construction method of RFF1 cells

A construction method and cell technology, applied to animal cells, other methods of inserting foreign genetic materials, vertebrate cells, etc., can solve the problems of high precision, high killing rate, and inability to balance immune cell attack and defense, so as to improve adaptability , high safety effect

Inactive Publication Date: 2019-08-16
BEIJING DCTY BIOTECH CO LTD
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Problems solved by technology

Through the combination of peptide shock and in vitro co-cultivation, ordinary T cells are transformed into super T cells with both offensive and defensive capabilities, which solves the problem that immune cells cannot balance offense and defense in cellular immunotherapy, with good precision and high killing rate

Method used

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  • Construction method of RFF1 cells
  • Construction method of RFF1 cells
  • Construction method of RFF1 cells

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Embodiment Construction

[0050] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0051] Tumor cell immunotherapy is an emerging tumor treatment modality. In terms of tumor cell immunotherapy, the existing LAK cells, DC cells, CIK cells, and DC-CIK cells have basically been proven to be ineffective. The embodiment of the present invention provides a new T cell for cellular immunotherapy.

[0052] The embodiment of the present invention provides a method for constructing RFF1 cells, wherein the method for preparing RFF1 cells ...

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Abstract

The invention provides a construction method of RFF1 cells and belongs to the technical field of biology, aiming to construct a kind of RFF1 cells for cellular immunotherapy, which are super T cells with both attack and defense, with good precision and high killing rate. The construction method includes the steps of loading tumor mutated polypeptides on PBMC (peripheral blood mononuclear cell) andthen performing polypeptide shocks on the PBMC loaded with the polypeptides; performing expanding culture after shocks to obtain EF cells; taking the tumor mutated polypeptides as antigen to direct stimulate the EF cells to screen precise polypeptides; knocking out immunosuppressive signal molecules on the PBNC based on the CRISPR (clustered regularly interspaced short palindromic repeats) technique; loading the PBMC with the precise polypeptides which are mixed with the PBMC knocking out the immunosuppressive signal molecules for co-culture, and during the co-culture process, performing multiple shocks with the precise polypeptides to obtain the RFF1 cells. The construction method can be applied to the cellular immunotherapy technique.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for constructing RFF1 cells, that is, a method for constructing T cells for cellular immunotherapy. Background technique [0002] Tumor cell immunotherapy is an emerging tumor treatment model. It collects immune cells from the patient, then cultures and expands them in vitro, and then infuses them back into the patient to stimulate and enhance the body's autoimmune function to treat tumors. Tumor cell immunotherapy is the fourth tumor treatment method after surgery, radiotherapy and chemotherapy. [0003] Normal or bioengineered human cells are transplanted or infused into patients, and the newly infused cells can replace damaged cells, or have stronger immune killing functions, so as to achieve the purpose of treating diseases. [0004] The human cells transformed by bioengineering are transformed in the process of culturing in vitro by special methods, which can e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/90C12N15/867C12N9/22C12N5/10
CPCC12N5/0636C12N9/22C12N15/86C12N15/907C12N2510/00C12N2740/15043
Inventor 焦顺昌张嵘周子珊解佳森王海燕李营营
Owner BEIJING DCTY BIOTECH CO LTD
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