Construction method of RFF1 cells

Abstract

The invention provides a construction method of RFF1 cells and belongs to the technical field of biology, aiming to construct a kind of RFF1 cells for cellular immunotherapy, which are super T cells with both attack and defense, with good precision and high killing rate. The construction method includes the steps of loading tumor mutated polypeptides on PBMC (peripheral blood mononuclear cell) andthen performing polypeptide shocks on the PBMC loaded with the polypeptides; performing expanding culture after shocks to obtain EF cells; taking the tumor mutated polypeptides as antigen to direct stimulate the EF cells to screen precise polypeptides; knocking out immunosuppressive signal molecules on the PBNC based on the CRISPR (clustered regularly interspaced short palindromic repeats) technique; loading the PBMC with the precise polypeptides which are mixed with the PBMC knocking out the immunosuppressive signal molecules for co-culture, and during the co-culture process, performing multiple shocks with the precise polypeptides to obtain the RFF1 cells. The construction method can be applied to the cellular immunotherapy technique.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for constructing RFF1 cells, that is, a method for constructing T cells for cellular immunotherapy. Background technique [0002] Tumor cell immunotherapy is an emerging tumor treatment model. It collects immune cells from the patient, then cultures and expands them in vitro, and then infuses them back into the patient to stimulate and enhance the body's autoimmune function to treat tumors. Tumor cell immunotherapy is the fourth tumor treatment method after surgery, radiotherapy and chemotherapy. [0003] Normal or bioengineered human cells are transplanted or infused into patients, and the newly infused cells can replace damaged cells, or have stronger immune killing functions, so as to achieve the purpose of treating diseases. [0004] The human cells transformed by bioengineering are transformed in the process of culturing in vitro by special methods, which can e...

AI Technical Summary

Problems solved by technology

Through the combination of peptide shock and in vitro co-cultivation, ordinary T cells are transformed into super T cells with both offensive and defensive capabilities, which solves the problem that immune cells cannot balance offense and defense in cellular immunotherapy, with good precision and high killing rate

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