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Fallopian tube cancer target antigen, CTL cell cultured by stimulation of fallopian tube cancer target antigen and application of fallopian tube cancer target antigen

A technology for stimulating and culturing fallopian tube cancer, which is applied in the field of tumor treatment and can solve the problems of inability to meet the individualization and variability of tumors

Inactive Publication Date: 2021-09-14
BEIJING DCTY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the promotion of precision therapy, conventional chemotherapy, radiotherapy and targeting can no longer meet the individuality and variability of tumors. Therefore, finding personalized tumor neoantigens and cultivating CTLs that recognize personalized tumor neoantigens will be a complete eradication. effective treatment for tumors

Method used

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  • Fallopian tube cancer target antigen, CTL cell cultured by stimulation of fallopian tube cancer target antigen and application of fallopian tube cancer target antigen
  • Fallopian tube cancer target antigen, CTL cell cultured by stimulation of fallopian tube cancer target antigen and application of fallopian tube cancer target antigen
  • Fallopian tube cancer target antigen, CTL cell cultured by stimulation of fallopian tube cancer target antigen and application of fallopian tube cancer target antigen

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preparation example Construction

[0032] The present invention also provides the preparation method of said CTL cells, comprising the following steps: 1) adjusting the concentration of CTL cells to 2-3×10 5 cells / mL; 2) Mix the CTL cells after adjusting the cell concentration, IL-2 and the fallopian tube cancer target antigen combination to obtain a culture system; 3) culture the culture system to obtain the fallopian tube cancer target antigen combination stimulation culture CTL cells.

[0033] In the present invention, the CTL cells are preferably obtained by co-culturing PBMC cells and APC cells. In the present invention, the step of culturing the APC cells is preferably as follows: PBMC cells are resuspended and cultured with fallopian tube cancer target antigen solution to obtain them. In the present invention, the culture medium of the recovery culture is preferably 1640+10% (v / v) FBS; the time of the recovery culture is preferably 22~26h, more preferably 24h; the temperature of the recovery culture is ...

Embodiment 1

[0043] For the whole exome sequencing results of tumor tissue, the tumor neoantigen analysis was performed using ImmunoMining tumor individualized neoantigen analysis and prediction software. The software copyright registration number: 2019SR0130720. Dingcheng Peptide Source Bio-Information Technology Co., Ltd. obtained 50 predicted antigens; synthesized the predicted 50 antigens (synthesized by the Polypeptide Synthesis Department of Nanjing GenScript).

[0044] Table 1 Peptide sequence and corresponding number

[0045]

[0046]

[0047]

[0048] 1. Preparation of APC by co-culture:

[0049] 1) Resuscitated frozen PBMC (2.5×10 7 cells), dilute the freezing solution with 1640+10% FBS, centrifuge at 1500rpm for 5min, resuspend with 10mL 1640+10%FBS, 1×10 6 cells / mL, 37°C, 5% CO 2 Recovery culture for 24h;

[0050] 2) Antigen polypeptide solutions were prepared separately, 1640+10% FBS+200U / mL IL-2+1600U / mL GM-CSF, the final concentration of each antigen polypeptide...

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Abstract

The invention provides a fallopian tube cancer target antigen, a CTL cell cultured by stimulation of the fallopian tube cancer target antigen and application of the fallopian tube cancer target antigen, and belongs to the technical field of tumor treatment. An amino acid sequence of the fallopian tube cancer target antigen is as shown in SEQ ID No.8; the fallopian tube cancer target antigen is an effective tumor antigen obtained through predictive analysis and screening on the basis of a whole exon detection result of tumor tissue of a patient, and the CTL cell cultured by the fallopian tube cancer target antigen has a specific killing effect on tumors; and the fallopian tube cancer target antigen provided by the invention can be applied to treatment of fallopian tube cancer.

Description

[0001] This application is a divisional application with an application date of March 18, 2020, an application number of 202010190785.9, and an invention title of "A Fallopian Tube Cancer Target Antigen Combination, CTL Cells Stimulated and Cultured by the Fallopian Tube Cancer Target Antigen Combination and Its Application". technical field [0002] The invention belongs to the technical field of tumor treatment, and in particular relates to a fallopian tube cancer target antigen combination, CTL cells stimulated and cultured by the fallopian tube cancer target antigen combination and application thereof. Background technique [0003] Primary fallopian tube cancer is a rare malignant tumor of the female reproductive tract. Its incidence rate accounts for 0.5% of gynecological malignancies. Most of them are 40 to 65 years old, and most of them occur in postmenopausal women. At present, the principle of treatment for fallopian tube cancer is based on surgery, combined with a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/74C12N5/0783A61K39/00A61P35/00
CPCC12N5/0638A61P35/00C12N2501/2302A61K2039/5158C07K14/4748A61K39/0011Y02A50/30
Inventor 焦顺昌张嵘王海燕
Owner BEIJING DCTY BIOTECH CO LTD
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