Breast cancer target antigen, CTL cell cultured by breast cancer target antigen in stimulated manner and application of CTL cell

A technology to stimulate culture and breast cancer, applied in the field of tumor treatment, can solve the problem of inability to meet the individuality and variability of tumors

Inactive Publication Date: 2021-09-14
BEIJING DCTY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] With the promotion of precision therapy, conventional chemotherapy, radiotherapy, and targeting can no longer meet the individuality and variability of tumors. Therefore, finding personalized tumor neoantigens and cultivating CTLs that recognize personalized tumor neoantigens will be a complete eradication effective treatment for tumors

Method used

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  • Breast cancer target antigen, CTL cell cultured by breast cancer target antigen in stimulated manner and application of CTL cell
  • Breast cancer target antigen, CTL cell cultured by breast cancer target antigen in stimulated manner and application of CTL cell
  • Breast cancer target antigen, CTL cell cultured by breast cancer target antigen in stimulated manner and application of CTL cell

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preparation example Construction

[0031] The present invention also provides the preparation method of said CTL cells, comprising the following steps: 1) adjusting the concentration of CTL cells to 2-3×10 5 cells / mL; 2) Mix the CTL cells after adjusting the cell concentration, IL-2 and the breast cancer target antigen combination to obtain a culture system; 3) culture the culture system to obtain the breast cancer target antigen combination stimulation culture CTL cells.

[0032] In the present invention, the CTL cells are preferably obtained by co-culturing PBMC cells and APC cells. In the present invention, the step of culturing the APC cells is preferably as follows: the PBMC cells are resuspended and cultured with breast cancer target antigen solution to obtain them. In the present invention, the culture medium of the recovery culture is preferably 1640+10% (v / v) FBS; the time of the recovery culture is preferably 22~26h, more preferably 24h; the temperature of the recovery culture is preferably 37 DEG C...

Embodiment 1

[0042] For the whole exome sequencing results of tumor tissue, the tumor neoantigen analysis was performed using ImmunoMining tumor individualized neoantigen analysis and prediction software. The software copyright registration number: 2019SR0130720. Dingcheng Peptide Source Bio-Information Technology Co., Ltd. obtained 17 predicted antigens; synthesized the predicted 17 antigens (synthesized by Nanjing KingScript Polypeptide Synthesis Department).

[0043] Table 1 Peptide sequence and corresponding number

[0044] Peptide number MT Epitope Seq 1 LLLTKTDI (SEQ ID No. 1) 2 TKTDIENLT (SEQ ID No. 2) 3 LLTKTDIEN (SEQ ID No. 3) 4 LTKTDIENL (SEQ ID No. 4) 5 TDIENLTIS (SEQ ID No. 5) 6 YLLLTKTD (SEQ ID No. 6) 7 WDGEDQTQD (SEQ ID No. 7) 8 EITLTWQW (SEQ ID No. 8) 9 ITLTWQWD (SEQ ID No. 9) 10 WQWDGEDQT (SEQ ID No. 10) 11 QWDGEDQTQ (SEQ ID No. 11) 12 TVCRHNYE (SEQ ID No. 12) 13 ERAAVDTVC (SEQ ID No. 1...

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Abstract

The invention provides a breast cancer target antigen, a CTL cell cultured by the breast cancer target antigen in a stimulated manner and application of the CTL cell, and belongs to the technical field of tumor treatment. The amino acid sequence of the breast cancer target antigen is shown as SEQ ID No. 1. The breast cancer target antigen is an effective tumor antigen obtained by predictive analysis and screening on the basis of total exon detection results of tumor tissues of patients, and the CTL cell cultured by the breast cancer target antigen has a specific killing effect on tumors. The breast cancer target antigen can be applied to treating breast cancer.

Description

[0001] This application is a divisional application with an application date of March 18, 2020, an application number of 202010190790.X, and an invention title of "A Combination of Breast Cancer Target Antigens, CTL Cells Stimulated and Cultured by a Combination of Breast Cancer Target Antigens and Its Application" . technical field [0002] The invention belongs to the technical field of tumor treatment, and in particular relates to a combination of breast cancer target antigens, CTL cells stimulated and cultured by the combination of breast cancer target antigens and applications thereof. Background technique [0003] The female mammary gland is composed of skin, fibrous tissue, mammary glands and fat. Breast cancer is a malignant tumor that occurs in the mammary gland epithelial tissue. 99% of breast cancers occur in women and only 1% in men. [0004] With the continuous deepening of the understanding of the biological behavior of breast cancer, as well as the transforma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12N5/0783A61K39/00A61P15/14A61P35/00
CPCC07K7/06C12N5/0638A61K39/0011A61P35/00A61P15/14C12N2501/999A61K2039/5158
Inventor 焦顺昌张嵘王海燕
Owner BEIJING DCTY BIOTECH CO LTD
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