Construction method of RFF2 cells

A construction method and cell technology, applied in the field of RFF2 cell construction, can solve the problem that immune cells cannot balance attack and defense, and achieve the effects of improving self-protection ability, improving adaptability and high safety.

Active Publication Date: 2019-08-16
BEIJING DCTY BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Through the combination of polypeptide impact, in vitro expansion of cells, and in vitro sealing, a kind of T cell with specific killing effect and integrated attack and defense is provided, which solves the problem that immune cells cannot balance attack and defense in cellular immunotherapy, with good precision and high killing rate

Method used

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  • Construction method of RFF2 cells
  • Construction method of RFF2 cells
  • Construction method of RFF2 cells

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Embodiment Construction

[0044] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0045] Such as figure 1 As shown, the embodiment of the present invention provides a method for constructing RFF2 cells, which is used to construct a specific T cell for cellular immunotherapy, named RFF2 cells. The method comprises the steps of:

[0046] S1) PBMC cells (peripheral blood mononuclear cells, peripheral blood mononuclear cells) are loaded with tumor-causing mutation polypeptides, and then the PBMC cells loaded with polypeptides are...

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Abstract

The invention discloses a construction method of RFF2 cells and belongs to the technical field of biology, aiming to construct a kind of specific T cells with both attack and defense and specific killing effect, which are the RFF2 cells used for cellular immunotherapy, with good precision and high killing rate. The construction method includes: loading tumor mutated polypeptides on PBMC (peripheral blood mononuclear cell) and then performing polypeptide shocks on the PBMC loaded with the polypeptides; performing expanding culture after shocks to obtain EF cells; taking the tumor mutated polypeptides as antigen to direct stimulate the EF cells to screen precise polypeptides; loading the PBMC with the precise polypeptides for culture, and during the culture process, performing multiple shocks with the precise polypeptides; performing continuous culture after shocks to obtain the EF cells; blocking immunosuppressive signal molecules of the RFF cells in vitro to obtain the RFF2 cells withPD1 and other antibodies.The construction method can be applied to the cellular immunotherapy technique.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing RFF2 cells. Background technique [0002] Tumor cell immunotherapy is an emerging tumor treatment model. It collects immune cells from the patient, then cultures and expands them in vitro, and then infuses them back into the patient to stimulate and enhance the body's autoimmune function to treat tumors. Tumor cell immunotherapy is the fourth tumor treatment method after surgery, radiotherapy and chemotherapy. [0003] Normal or bioengineered human cells are transplanted or infused into patients, and the newly infused cells can replace damaged cells, or have stronger immune killing functions, so as to achieve the purpose of treating diseases. [0004] The bioengineered cells are modified by special methods in the in vitro culture process, which can improve their lethality and effectively kill tumor cells in patients. For example, Chinese patent application...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783C12N1/38
CPCC12N1/38C12N5/0638
Inventor 焦顺昌张嵘周子珊解佳森王海燕李营营
Owner BEIJING DCTY BIOTECH CO LTD
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