A method for constructing an animal model of psoriasis

A technology for animal models and construction methods, which can be used in pharmaceutical formulations, drug combinations, skin diseases, etc., and can solve problems such as short maintenance time of models.

Active Publication Date: 2021-02-26
北京市中医研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern medicine has defined psoriasis as a typical psychosomatic disease, but the current animal model of psoriasis does not reflect this clinical reality, it can only show simple skin lesions, and the model lasts for a short time

Method used

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  • A method for constructing an animal model of psoriasis
  • A method for constructing an animal model of psoriasis
  • A method for constructing an animal model of psoriasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Selection and feeding of experimental animals: male SPF grade BALB / c mice, 6-8 weeks old, body weight (20±2) g, China Food and Drug Control Institute, animal license number SCXK (Beijing) 20140013. Mice were kept in a ventilated and clean animal room with controlled room temperature (20±2)°C, relative humidity (40-70)%, 12h light and 12h dark cycles, and all mice were free to eat except during emotional stress , drinking water.

[0059] The mice were acclimatized to the new environment in a single cage for 1 week, and during this period, the animals were touched every day (3min / d) to reduce the impact of operation. One week later, the mice were trained to drink water at 9:00-9:10 and 21:00-21:10 every day, and no water was given during the two training periods. After one week of training, change to random empty bottle stimulation, that is, water bottles with water and no water are randomly assigned to each mouse. The drinking time and drinking time are the same as duri...

Embodiment 2

[0066] Selection and feeding of experimental animals: male SPF grade BALB / c mice, 6-8 weeks old, body weight (20±2) g, China Food and Drug Control Institute, animal license number SCXK (Beijing) 20140013. Mice were kept in a ventilated and clean animal room with controlled room temperature (20±2)°C, relative humidity (40-70)%, 12h light and 12h dark cycles, and all mice were free to eat except during emotional stress , drinking water.

[0067] The mice were acclimatized to the new environment in a single cage for 1 week, and during this period, the animals were touched every day (3min / d) to reduce the impact of operation. One week later, the mice were trained to drink water at 9:00-9:10 and 21:00-21:10 every day, and no water was given during the two training periods. After one week of training, change to random empty bottle stimulation, that is, water bottles with water and no water are randomly assigned to each mouse. The drinking time and drinking time are the same as duri...

experiment example 1

[0071] Behavioral experiments.

[0072] 1.1 Elevated Plus Maze

[0073] A blank control group is set on the basis of Example 1, that is, no empty bottle stimulation training is carried out, and the rest are the same as in Example 1. The results are shown in figure 1 and figure 2 .

[0074] figure 1 Shown in the blank control group (group C) that the number of times (frequency) and time of entering the open arm in the elevated cross is significantly higher than that of the empty bottle stimulation training group (ES group), figure 2 Its statistical result graph (left: time comparison graph; right: frequency comparison graph). It shows that mice in ES group have obvious anxiety-like behavior.

[0075] 1.2 Open field experiment

[0076] Carry out comparative experiment setting and experiment by experimental example 1 and embodiment 1, the results are shown in image 3 and Figure 4 .

[0077] figure 1 The results showed that the activity time and distance of the mice ...

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Abstract

The invention relates to the field of animal models, in particular to a construction method of a psoriasis animal model. The construction method comprises the application of imiquimod to induce psoriasis-like lesions in an anxious animal. The constructed mice can not only reflect the anxiety state of clinical patients, but also aggravate the psoriasis-like lesions, and prolong the maintenance timeof psoriasis-like lesions induced by imiquimod.

Description

technical field [0001] The invention relates to the field of animal models, in particular to a method for constructing psoriasis animal models. Background technique [0002] Psoriasis (Psoriasis), commonly known as "psoriasis", is a common chronic inflammatory skin disease characterized by abnormal activation of lymphocytes, abnormal proliferation and differentiation of keratinocytes, and infiltration of inflammatory cells. , the main histopathological features include: hypokeratosis or hyperkeratosis of intraepidermal cells, enlarged and distorted blood vessels in the dermis, infiltration of various inflammatory cells, etc. Because of its long course of disease and extensive skin lesions, it often has a serious impact on the physical and mental health of patients. [0003] An important tool for studying the etiology, pathogenesis, and treatment of human diseases is animal models, but psoriasis is rare in any non-human animals, and naturally occurring cases are extremely ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4745A61P17/06
CPCA61K31/4745A61P17/06
Inventor 张璐李萍王燕赵京霞李宁飞底婷婷付京蒙玉娇郭简宁
Owner 北京市中医研究所
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