Control method of a section-type thermal convection PCR instrumen

Abstract

The invention relates to a control method of a section-type thermal convection PCR instrument, which sets a temperature value of DNA denaturation reaction; Setting the temperature value above the heater; Controlling the temperature above the heater to be maintained within a set temperature value by a constant temperature control module; a temperature sensor detect an instant temperature signal ofthat heater, convert the instant temperature signal into an instant resistance signal through a temperature transmitter and outputs the instant resistance signal to an analog-to-digital convert on a control module for converting the instant current signal. The analog-to-digital converter outputs the instant current signal to the PID control module. The PID control module compares the instant current signal with the current value corresponding to the set DNA denaturation reaction temperature value to obtain the difference value. The PID control module sends a modulation signal to the electromagnetic relay according to the difference value, and the electromagnetic relay adjusts the current of the silica gel heating sheet according to the modulation signal, so that the temperature of the heater tends to set the temperature value of DNA denaturation reaction.

Description

technical field [0001] The invention belongs to the field of molecular biology for amplifying and amplifying specific DNA fragments, in particular to a control method for a one-stage thermal convection PCR instrument for amplifying and amplifying specific DNA fragments. Background technique [0002] The PCR reaction needs to go through three stages of DNA denaturation, annealing, and extension. Each stage corresponds to a different temperature. Among them, denaturation is the use of DNA that will become single-stranded when the temperature is about 95°C in vitro, and annealing is the use of DNA at a temperature of about 60°C. , The primer and the single strand are combined according to the principle of complementary base pairing, and the extension is the process of synthesizing the complementary strand along the direction from the phosphate to the five-carbon sugar under the action of the polymerase at a temperature of about 70°C. [0003] The traditional thermal cycler repe...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a control method for a one-stage thermal convection PCR instrument, so as to solve the problem that the time required for the existing PCR instrument to reduce the volume and the entire process of amplification cannot meet the general promotion problem

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