One-stage heat convection PCR instrument and control method

Abstract

The invention relates to a one-stage heat convection PCR instrument, which includes a temperature control system, a heating system, a control module and a power supply. According to a technical schemeadopted by the invention, the one-stage heat convection PCR instrument utilizes an aluminum alloy block heating plate at the bottom of the instrument for heating, the capillary bottom temperature iscontrolled at a denaturation temperature, and the capillary top temperature is controlled at an annealing temperature. Under the action of gravity difference and temperature difference, the reagent inthe capillary can reach different temperatures constantly through natural convection, thus realizing PCR. The temperature distribution of the system varies smoothly along the capillary length, and different PCR steps can be carried out simultaneously. In the simple system, DNA amplification can be accomplished without expensive and precise thermal cycler, and no additional complex hardware is needed.

Description

technical field [0001] The invention belongs to the field of molecular biology for amplifying and amplifying specific DNA fragments, in particular to a one-stage thermal convection PCR instrument and a control method for amplifying and amplifying specific DNA fragments. Background technique [0002] The PCR reaction needs to go through three stages of DNA denaturation, annealing, and extension. Each stage corresponds to a different temperature. Among them, denaturation is the use of DNA that will become single-stranded when the temperature is about 95°C in vitro, and annealing is the use of DNA at a temperature of about 60°C. , The primer and the single strand are combined according to the principle of complementary base pairing, and the extension is the process of synthesizing the complementary strand along the direction from the phosphate to the five-carbon sugar under the action of the polymerase at a temperature of about 70°C. [0003] The traditional thermal cycler repe...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a one-stage thermal convection PCR instrument and its control method, so as to solve the problem that the existing PCR instrument cannot meet the general promotion in the volume reduction and the time required for the whole process of amplification.

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