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Dead wood trunk injection bacteria solution for preventing and controlling dendroctonus armandi and preparation method thereof

A technique of beetle-injection and bacterial liquid, which is applied in the field of plant protection, can solve the problems of inconvenient carrying, low efficiency, and aggravated damage in the affected area, and achieve the effect of saving manpower and material resources, significant effect, and long-term control

Inactive Publication Date: 2019-01-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the bark beetle led by the Huashan pine beetle has continued to occur in the Bashan forest area of ​​the Qinling Mountains, which has induced the invasion of other pests and caused huge economic and ecological losses.
[0003] In recent years, chemical control, biological control and pheromone application have been mainly adopted around the Huashan pine beetle. Chemical control usually chooses emamectin benzoate, omethoate, dichlorvos, imidacloprid and dinotefuran, etc. The method of spraying is to mix a variety of chemicals to enhance the control effect. Sometimes it is necessary to plan the bark for spraying or spray the damaged wood after cutting the bark. It is limited by the climate and the area where the Huashan pine beetle occurs. Restricted by geographical factors, there are many disadvantages in chemical spraying, which not only consumes a lot of manpower and material resources, but also pollutes the environment; biological control is to choose to spray Beauveria bassiana, Metarhizium anisopliae, etc., but it needs to carry facilities to spray. It is extremely inconvenient to carry in the forest environment, and the efficiency is low. Once it rains, the Beauveria bassiana sprayed on the trunk will be washed away by the rain, and it will not work at all; the application of pheromones can choose attractants to trap and repellants Isolation and other methods are widely used at present, but the objective existence of beetles is not proportional to the actual number that can be lured, which makes the damage of the affected area still aggravated
Therefore, any single prevention and treatment is not obviously effective at present, and it is necessary to use high-efficiency agents to implement comprehensive prevention and control in combination with the actual situation in the prevention and control area.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Preventing and treating dead wood of beetles The dried bacterial solution is formed by the following components and parts by weight:

[0016] Nutrient solution 64, Tween-80 12, Beauveria bassiana 24;

[0017] The preparation method of the above-mentioned prevention and treatment of the dead wood injection dry bacterial solution of the large beetle is as follows:

[0018] a. Simply mix the components of the nutrient solution to prepare the nutrient solution,

[0019] b. Add Tween-80 to the nutrient solution, and measure the pH value to be 6.5,

[0020] c. Add Beauveria bassiana at the end, keep the temperature at 25°C,

[0021] d. Divide the prepared bacterial solution into pointed plastic bottles for storage.

Embodiment 2

[0022] Embodiment two: prevent and treat the dead wood of beetles. The dry bacterial solution is formed by the following components and parts by weight:

[0023] Nutrient solution 70, sodium alginate 10, Beauveria bassiana 10, Beauveria bassiana oospor 10;

[0024] The preparation method of the above-mentioned prevention and treatment of the dead wood injection dry bacterial solution of the large beetle is as follows:

[0025] a. Simply mix the components of the nutrient solution to prepare the nutrient solution,

[0026] b. Add sodium alginate to the nutrient solution, and measure the pH value to be 6.5.

[0027] c. Add Beauveria bassiana and Beauveria bassiana at the end, keep the temperature at 25°C,

[0028] d. Divide the prepared bacterial solution into pointed plastic bottles for storage.

Embodiment 3

[0029] Embodiment three: prevent and treat the dead wood of beetles. The dry bacterial solution is formed by the following components and parts by weight:

[0030] Nutrient solution 65, sodium alginate 5, Beauveria bassiana 20, Beauveria bassiana oospor 10;

[0031] The preparation method of injecting dry bacterial liquid for preventing and treating dead wood beetles is as follows:

[0032] a. Simply mix the components of the nutrient solution to prepare the nutrient solution,

[0033] b. Add sodium alginate to the nutrient solution, and measure the pH value to be 6.3.

[0034] c. Finally, add Beauveria bassiana and Beauveria bassiana, and keep the temperature at 28°C.

[0035] d. Divide the prepared bacterial solution into pointed plastic bottles for storage.

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PUM

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Abstract

The invention provides a dead wood trunk injection bacteria solution for preventing and controlling dendroctonus armandi. The dead wood trunk injection bacteria solution is prepared from the followingcomponents in parts by weight: 60-78 parts of a nutrient solution, 5-17 parts of a stabilizer, and 20-30 parts of beauveria bassiana. Pinus armandii withering wood is used as a control object, the bacteria solution is immobilized by adopting a trunk injection way, a bactericide is not adsorbed, and slowly flows outward and enters bark cracks, and spores can spread on a whole trunk, so that dendroctonus armandi on the trunk can be killed, and meanwhile, the trunk is used as a spore spreading body, and a large amount of spores quickly spread in a whole forest, so that the diffusion of dendroctonus armandi is effectively controlled; and the dead wood trunk injection bacteria solution has the advantages of remarkable pest control effect, stable quality, long-acting prevention, no weather influence and low cost, saves manpower and material resources, provides a new way for preventing dendroctonus armandi and other trunk-boring pests, and can be popularized and applied in vast forest zones.

Description

technical field [0001] The invention relates to an insecticide and a preparation method, in particular to a dried bacterial liquid for preventing and treating dead wood of beetles and a preparation method, belonging to the field of plant protection. Background technique [0002] Huashan pine is named for its concentrated origin in Huashan, Shaanxi, my country. Its distribution area is located in the vast area where the western plateau transitions to the eastern plains and low mountains and hills, mostly in mountainous areas. It is one of the main constituent tree species and one of the reforestation tree species. It is also a unique tree species in my country. It has the characteristics of rapid growth and excellent material, and has important economic and ecological values. In recent years, bark beetles led by the Huashan pine beetle continue to occur in the Bashan forest area of ​​the Qinling Mountains, which in turn induces the invasion of other pests, causing huge economi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/04
CPCA01N63/30
Inventor 唐明宁航陈辉代鲁鲁
Owner SOUTH CHINA AGRI UNIV
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