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RFF2 cell

A technology of RFF2 and cells, which is applied in the direction of animal cells, vertebrate cells, cell culture active agents, etc., can solve the problem that immune cells cannot balance attack and defense, and achieve the effect of improving self-defense ability, improving adaptability, and high safety

Active Publication Date: 2019-02-01
BEIJING DCTY BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to provide a new specific T cell (named as RFF2 cell) for cellular immunotherapy, combined with polypeptide impact, in vitro expansion of cells, and in vitro sealing, to provide a specific killing effect and integrated attack and defense. T cells solve the problem that immune cells cannot balance offense and defense in cellular immunotherapy, with good precision and high killing rate

Method used

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Embodiment Construction

[0044] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0045] Such as figure 1 As shown, the embodiment of the present invention provides a specific T cell for cellular immunotherapy, named RFF2 cell. The preparation method of the RFF2 cell comprises the following steps:

[0046] S1) PBMC cells (peripheral blood mononuclear cells, peripheral blood mononuclear cells) are loaded with polypeptides that cause tumor mutations, and then a polypeptide shock is performed on the PBMC cells loaded with polype...

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Abstract

The invention provides an RFF2 cell, and belongs to the field of biotechnologies. The RFF2 cell used for cellular immunotherapy, provided by the invention, is a defensive and offensive super T cell with a specific killing effect, and has a high accuracy and a high killing rate. A method for preparing the RFF2 cell comprises the following steps: a PBMC is loaded with a tumor mutation-causing polypeptide, and the polypeptide loaded PBMC is subjected to polypeptide impact; amplification culture is carried out after impact to obtain an FF cell; the tumor mutation-causing polypeptide is used as anantigen to directly stimulate the FF cell in order to screen an accurate polypeptide; the PBMC is loaded on the accurate polypeptide, and the loaded PBMC is cultured, and is impacted with the accuratepolypeptide multiple times during the co-culturing; the impacted PBMC is continuously cultured to obtain an RFF cell; and the RFF cell undergoes immunosuppressive signal molecule in-vitro blocking byusing PD1 and other antibodies in order to obtain the RFF1 cell. The RFF2 cell can be used in a cellular immunotherapy technique.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an RFF2 cell used for cellular immunotherapy and a preparation method thereof. Background technique [0002] Tumor cell immunotherapy is an emerging tumor treatment model. It collects immune cells from the patient, then cultures and expands them in vitro, and then infuses them back into the patient to stimulate and enhance the body's autoimmune function to treat tumors. Tumor cell immunotherapy is the fourth tumor treatment method after surgery, radiotherapy and chemotherapy. [0003] Normal or bioengineered human cells are transplanted or infused into patients, and the newly infused cells can replace damaged cells, or have stronger immune killing functions, so as to achieve the purpose of treating diseases. [0004] The bioengineered cells are modified by special methods in the in vitro culture process, which can improve their lethality and effectively kill tumor cells in patients....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783A61P35/00
CPCA61P35/00C12N5/0636C12N2501/51C12N2501/998
Inventor 焦顺昌张嵘周子珊解佳森王海燕李营营
Owner BEIJING DCTY BIOTECH CO LTD
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