Method for treating crop seeds through atmospheric pressure room temperature plasma
A plasma and crop technology, which is applied in the field of plasma treatment of plant seeds, can solve the problems of few mutation sites, high cost, insignificant treatment effect, etc., and achieve the effect of improving germination rate and better mutagenesis effect.
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Embodiment 1
[0021] A method for treating crop seeds with atmospheric pressure and room temperature plasma, the specific operation steps are as follows:
[0022] (1) For the obtained mung bean, soybean, corn and wheat seeds (the original seeds that have not been genetically modified or treated by other mutagenic means), the experimental samples are selected from seeds with intact seed coats and uniform particle size, and use 10% Sodium hypochlorite solution for surface disinfection;
[0023] (2) 300 seeds of each crop were selected for experimentation, and grouped into 6 groups, each with 50 seeds;
[0024] (3) Spread the seeds of the experimental group 3-6 in step (2) on the plate respectively, place it directly under the plasma irradiation source at room temperature at normal pressure, set the gas volume to 20-40L / min, and the irradiation time to 10-60min ;
[0025] (4) Start the plant-type atmospheric pressure and room temperature plasma mutation breeding instrument to release the pla...
Embodiment 2
[0030] A method for treating crop seeds with atmospheric pressure and room temperature plasma, the specific operation steps are as follows:
[0031] (1) For the obtained mung bean, soybean, corn and wheat seeds (the original seeds that have not been genetically modified or treated by other mutagenic means), the experimental samples are selected from seeds with intact seed coats and uniform particle size, and use 10% Sodium hypochlorite solution for surface disinfection;
[0032] (2) 300 seeds of each crop were selected for experimentation, grouped according to Table 1, and divided into 6 groups, 50 seeds in each group;
[0033]
[0034] (3) Spread the seeds of experimental groups 3-6 in step (2) on a flat plate respectively, place them directly under the plasma irradiation source at room temperature at normal pressure, set the gas volume to 40L / min, and the irradiation time to 60min;
[0035] (4) Start the plant-type atmospheric pressure and room temperature plasma mutatio...
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