Method for treating crop seeds through atmospheric pressure room temperature plasma

A plasma and crop technology, which is applied in the field of plasma treatment of plant seeds, can solve the problems of few mutation sites, high cost, insignificant treatment effect, etc., and achieve the effect of improving germination rate and better mutagenesis effect.

Inactive Publication Date: 2019-03-15
LUOYANG TMAXTREE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, radiation mutagenesis and chemical mutagenesis not only have operational safety problems, but also have greater genotoxicity; space mutagenesi...

Method used

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  • Method for treating crop seeds through atmospheric pressure room temperature plasma
  • Method for treating crop seeds through atmospheric pressure room temperature plasma
  • Method for treating crop seeds through atmospheric pressure room temperature plasma

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Experimental program
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Embodiment 1

[0021] A method for treating crop seeds with atmospheric pressure and room temperature plasma, the specific operation steps are as follows:

[0022] (1) For the obtained mung bean, soybean, corn and wheat seeds (the original seeds that have not been genetically modified or treated by other mutagenic means), the experimental samples are selected from seeds with intact seed coats and uniform particle size, and use 10% Sodium hypochlorite solution for surface disinfection;

[0023] (2) 300 seeds of each crop were selected for experimentation, and grouped into 6 groups, each with 50 seeds;

[0024] (3) Spread the seeds of the experimental group 3-6 in step (2) on the plate respectively, place it directly under the plasma irradiation source at room temperature at normal pressure, set the gas volume to 20-40L / min, and the irradiation time to 10-60min ;

[0025] (4) Start the plant-type atmospheric pressure and room temperature plasma mutation breeding instrument to release the pla...

Embodiment 2

[0030] A method for treating crop seeds with atmospheric pressure and room temperature plasma, the specific operation steps are as follows:

[0031] (1) For the obtained mung bean, soybean, corn and wheat seeds (the original seeds that have not been genetically modified or treated by other mutagenic means), the experimental samples are selected from seeds with intact seed coats and uniform particle size, and use 10% Sodium hypochlorite solution for surface disinfection;

[0032] (2) 300 seeds of each crop were selected for experimentation, grouped according to Table 1, and divided into 6 groups, 50 seeds in each group;

[0033]

[0034] (3) Spread the seeds of experimental groups 3-6 in step (2) on a flat plate respectively, place them directly under the plasma irradiation source at room temperature at normal pressure, set the gas volume to 40L / min, and the irradiation time to 60min;

[0035] (4) Start the plant-type atmospheric pressure and room temperature plasma mutatio...

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Abstract

The invention provides a method for treating crop seeds through atmospheric pressure room temperature plasma. The method can be used for treating mung bean seeds, soybean seeds, corn seeds and wheat seeds. Compared with a control group, the bud ratio of crop seeds treated through plasma is obviously improved; the bud ratio of crop seeds subjected to germination treatment is further obviously improved, and the mutagenic effect is better, for example, the bud ratio of crop seeds subjected to mechanical seed coat breaking treatment is obviously higher than that of crop seeds subjected to seed coat complete germination, and the bud ratio of crop seeds subjected to gibberellin soaking treatment is obviously higher than that of un-soaked crop seeds.

Description

technical field [0001] The invention belongs to the application field of plasma technology, and in particular relates to a method for treating plant seeds with plasma at normal pressure and room temperature. Background technique [0002] Under natural conditions, due to changes in the living environment and the instability of genetic material, the plant itself will undergo low-frequency mutations. Since the 20th century, humans have gradually mastered some mutagenesis methods to induce mutations in plants, thereby increasing the scope of species variation. At present, the methods mainly used in plant mutagenesis are: radiation mutagenesis, chemical mutagenesis, space mutagenesis, ion implantation mutagenesis, ultraviolet mutagenesis and plasma mutagenesis. Among them, radiation mutagenesis and chemical mutagenesis not only have operational safety problems, but also have greater genotoxicity; space mutagenesis and ion implantation process samples are more costly, and cause f...

Claims

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Application Information

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IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 王立言
Owner LUOYANG TMAXTREE BIOTECH CO LTD
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