Lactarius deliciosus spore separation and culture method

Abstract

The invention provides a lactarius deliciosus spore separation and culture method which comprises the following steps: (1) wild age-appropriate lactarius deliciosus is found in the wild, and transported to a laboratory by using an experimental cold closet; (2) the lactarius deliciosus is suspended in a prefabricated culture bottle, and spores are received by using a culture dish; (3) the temperature of a cultivation room is adjusted to be 25-30 DEG C, the illuminance is adjusted to 700-800 lux, after 4-5 small, the sexual spores of the lactarius deliciosus can be received 4-5 hours after; (4)single spores are separated under a microscope for culturing, and then the spores of the lactarius deliciosus are inoculated on a culture medium; (5) the cultivation temperature ranges from 17 DEG C to 20 DEG C, and mycelia stop growing at the temperature lower than 14 DEG C; the culturing humidity is adjusted to be 55-65%, other infectious microbes can be easily bred due to the fact that the humidity is too large, and due to the fact that the humidity is too low, the mycelia cultured in a test tube are easy to dehydrate and die. The method can be used for artificially culturing a lactarius deliciosus strain.

Description

technical field [0001] The invention relates to the technical field of edible fungi, in particular to a method for isolating and cultivating swan spores. Background technique [0002] Swan fungus is a kind of edible fungus. It is the best when the pine flowers fall to the ground during the cold dew. It is delicious and has a strange fragrance; root relationship. In the mountains and hills of Songzi, Pinus massoniana forests are widely distributed and the climate is humid, which is suitable for the growth of Cygnus swani. The pine forests in Baojing, Xiangxi, Hunan, Linli and Songzikou on the south bank of the Yangtze River have the most yield and the best quality. [0003] At present, the whole world has not been able to complete the artificial cultivation technology of Cygnus swani. The main reason is that the breeding of the mother species must select and breed the sexual spores before the fruiting body can be bred. Contents of the invention [0004] The purpose of the...

AI Technical Summary

Problems solved by technology

[0003] At present, the artificial cultivation technology of Cygnus swanensis has not been completed in the whole wor