Differential protein database for expressing kidney deficiency testis and construction method thereof

A technology of differential protein and construction method, applied in the field of proteomics, can solve the problems of few research technologies and methods, and the establishment of proteomics database is still in the blank stage

Inactive Publication Date: 2019-04-12
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0005] However, so far, there are few research techniques and methods that combine the "syndrome" of traditional Chinese medicine with molecular biology (especially proteomics), and the establishment of a proteomics database about the "syndrome" of traditional Chinese medicine is still in a blank stage

Method used

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  • Differential protein database for expressing kidney deficiency testis and construction method thereof
  • Differential protein database for expressing kidney deficiency testis and construction method thereof
  • Differential protein database for expressing kidney deficiency testis and construction method thereof

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Experimental program
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Embodiment

[0046] 1 Differential proteins expressed in the testes of mice with kidney yang deficiency

[0047] Compared with the normal group, a total of 87 differentially expressed proteins were identified in the testis of mice with kidney yang deficiency, of which 27 were up-regulated and 60 were down-regulated. (See Table 1)

[0048] Table 1 Differentially expressed proteins in the testis of mice with kidney yang deficiency

[0049]

[0050]

[0051]

[0052]

[0053] 2 Differential protein interaction network expressed in testis of mice with kidney yang deficiency

[0054] The 87 identified differential proteins of the testis of the kidney-yang deficiency group were entered into the STRING database, and when the comprehensive confidence level was ≥0.15, a protein interaction graph was formed with the 85 differential proteins as nodes, as shown in figure 2 (Circles represent genes, lines represent protein interactions between genes). When the comprehensive confidence i...

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Abstract

The invention relates to a differential protein database for expressing kidney deficiency testis and a construction method thereof. The differential protein database for expressing kidney deficiency testis is obtained by selecting a mode of deficiency of kidney-yin and a mode of deficiency of kidney-yang induced by hydrocortisone and comparing differential proteins of a normal model, the mode of deficiency of kidney-yin and the mode of deficiency of kidney-yang for expressing testis by means of an iTRAQ technology, a protein interaction network and a clustering analyzing means. Essence of deficiency of the kidney is explained by means of the differential protein database constructed, and the differential protein database provides a basis for diagnosing and treating deficiency of the kidneyin the aspect of proteomics and has very important meaning in diagnosing and treating male reproductive dysfunction.

Description

technical field [0001] The invention relates to the technical field of proteomics, in particular to a database of differential proteins expressed in testes with kidney deficiency and a construction method thereof. Background technique [0002] Proteomics takes the proteome as the research object to study the protein composition of cells, tissues or organisms and their changes. The study of proteomes can not only provide the material basis for the laws of life activities, but also clarify the mechanisms of many diseases. And overcome to provide theoretical basis and solutions. [0003] TCM includes different "syndrome", but based on the history and diagnosis and treatment characteristics of TCM, the field of TCM rarely diagnoses "syndrome" based on specific test reports and quantitative data, and "syndrome" is generally diagnosed through specific symptoms. Proteomics research technology can be used to identify the proteome of the body. If we analyze the overall protein compo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B50/00G16B20/00G16H50/20
CPCG16H50/20
Inventor 马静贾瑊陈昌波
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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