A method for screening drought-tolerant varieties by using the white root number of rice

Abstract

The invention discloses a method for screening drought-tolerant varieties by using the number of white roots of rice, which comprises the following steps: laying germination paper on a culture dish, evenly placing rice seeds of the same variety on the culture dish, and then placing the culture dish in an artificial climate Hydroponic cultivation was carried out in the box, half of the hydroponic rice seeds were used for drought treatment, and half were used as a control; when the hydroponic rice grew to the three-leaf one-heart stage, PEG-6000 was used to simulate drought, and the concentration of PEG was 20%. Since the addition of the PEG solution is the beginning of the drought treatment, record the time when the leaves of different varieties appear curled. When a variety appears curled leaves, stop the drought treatment; wash the roots of the obtained cultured seedlings, and put them into the root scanning system for scanning According to the species, the number of white roots in the room temperature control group and the drought treatment group were counted respectively; the drought tolerance index was calculated.

Description

technical field [0001] The invention relates to the field of rice genetic engineering, in particular to a method for screening drought-tolerant varieties by using the number of white roots of rice. Background technique [0002] Water shortage is a global problem currently restricting agricultural production. About 43% of the world's arable land is in arid and semi-arid areas. Drought stress seriously affects the growth and development of plants, resulting in crop yield reduction. my country is a water-poor country, and the contradiction between supply and demand of water resources is very sharp. In recent years, water shortage will be one of the most serious problems facing our country. Moreover, in agricultural production, frequent droughts often occur, causing huge losses to food production. The annual loss of food production caused by drought in the world is almost equal to the sum of losses caused by all other environmental factors. [0003] The annual loss of food pro...