A kind of disposable macroalgae double layer makeup remover and preparation method thereof
A technology of makeup remover and giant algae, which is applied in the field of no-wash double-layer makeup remover of giant algae and its preparation, can solve the problems of strong irritation and unsuitable makeup removal, and achieve the effect of good makeup removal effect, fast emulsification speed, and mild texture
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Embodiment 1
[0085] The preparation method of embodiment 1 macroalgae ferment
[0086] (1) Dry the macroalgae and beat it into macroalgae powder, add distilled water to mix, and then ultrasonically disperse and dissolve. Use a 0.22μm membrane filter to remove fat;
[0087] (2) continuously stirring the obtained macroalgae solution, and adding 1-5% NaOH solution by mass percentage therein to adjust its pH value;
[0088] (3) After stirring evenly, sterilize in an autoclave at 121° C. for 15 minutes, and then cool to 25° C. to obtain a macroalgae mixture;
[0089] (4) The culture solution is composed of the following components by weight percentage: 0.2% beef extract, 0.8% peptone, 0.5% sodium chloride, 2% agar, and 0.8% glucose. Adjust its pH value to 6.5-7.5 with 2% NaOH solution by mass percentage, then mix Bacillus subtilis, Bacillus licheniformis and Aspergillus niger in a certain ratio to form a mixed bacterium, and inoculate it in the culture solution;
[0090] (5) adding the nutri...
Embodiment 2
[0097] Example 2 Evaluation of free radical scavenging ability (in vitro DPPH method)
[0098] Accurately weigh 3.5mg of DPPH reagent, dissolve it with absolute ethanol, and quantitatively transfer it to a 10mL volumetric flask, dilute to the mark with absolute ethanol, take 2mL into a 100mL volumetric flask, and shake well to obtain a concentration of 0.0178mmol / L DPPH The solution was stored in the refrigerator for later use.
[0099] Accurately weigh 2 mg each of the dried macroalgae enzymes prepared by methods 1-11, dissolve them in absolute ethanol, and quantitatively transfer them to a 50mL volumetric flask, use absolute ethanol to measure to the mark, take 10mL into a 100mL volumetric flask, and shake well The obtained concentration is 4mg / L macroalgae enzyme test solution.
[0100] Add 4.0mL DPPH solution and 4.0mL macroalgae enzyme test solution in sequence to a 10mL colorimetric tube, then add absolute ethanol to the mark, mix well and immediately use a 1cm colorime...
Embodiment 3
[0104] Embodiment 3 Preparation of Macroalgae Oil Extract
[0105] (1) After drying the macroalgae, beat it into macroalgae powder, add distilled water to mix, and perform ultrasonic treatment in an ice bath;
[0106] (2) mixing the macroalgae solution obtained in step (1) with squalane, and extracting by shaking at a certain temperature;
[0107] (3) After the extraction is finished, add β-cyclodextrin in proportion to the obtained macroalgae mixture, and refine it by shaking at a certain temperature;
[0108] (4) Stand still for 2 hours after shaking, and perform centrifugation at a speed of 8000 r / min for 10 minutes.
[0109] (5) Filter the upper oil phase twice with a filter membrane with a specification of 0.22 μm to obtain macroalgae oil extract.
[0110] Table 3 The parameters of each preparation method of macroalgae oil extract
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