Microbial compound preparation for fishes and shrimps and preparation method of microbial compound preparation

A compound preparation and microorganism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of general biological characteristics, unstable effect, and unsustainable survival of resurrected strains, and enhance the mucosal barrier. Function and immune function, improve gastrointestinal function, improve absorption effect

Pending Publication Date: 2019-05-28
ZHANJIANG EVERGREEN CULTURE TECH SERVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the beneficial microorganisms used in aquaculture production are single strains. The biological properti

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of the microbial composite preparation for fish and shrimp comprises the following steps:

[0031] S1, 5% of the seed culture solution of Rhodopseudomonas palustris, 35% of the seed culture solution of Candida utilis, 40% of the seed culture solution of Enterococcus faecalis (high resistance), 10% of the seed culture solution of Lactobacillus acidophilus %, 4% of the seed culture solution of Bacillus subtilis, and 6% of the seed culture solution of Bacillus licheniformis; take the seed culture solution of each bacterial species according to the above ratio, and inoculate them into the same liquid medium under aseptic conditions respectively, The inoculum size is 2 to 5% of the weight of the liquid medium;

[0032] S2. Under the condition that the ratio of the volume of oxygen to the volume of fermentation broth in a fermenter with a dissolved oxygen of 5% is 5%, the airtight static culture at 28°C to 32°C for 7 to 15 days will reduce the pH value ...

Embodiment 2

[0043] The preparation method of the microbial composite preparation for fish and shrimp comprises the following steps:

[0044] S1, 15% of the seed culture solution of Rhodopseudomonas palustris, 25% of the seed culture solution of Candida utilis, 30% of the seed culture solution of Enterococcus faecalis (high resistance), 20% of the seed culture solution of Lactobacillus acidophilus %, 6% of the seed culture solution of Bacillus subtilis, and 4% of the seed culture solution of Bacillus licheniformis; take the seed culture solution of each bacterial species according to the above proportioning ratio, and inoculate them into the same liquid medium under aseptic conditions respectively, The inoculum size is 2~5% of the weight of the liquid culture medium; (the preparation method of the seed culture solution of each strain is the same as that of Example 1, and will not be repeated.)

[0045] S2. Under the condition that the ratio of the volume of oxygen to the volume of fermenta...

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PUM

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Abstract

The invention discloses a microbial compound preparation for fishes and shrimps and a preparation method of the microbial compound preparation. The compound preparation is prepared from seed culture solutions containing the following strains in percentage by weight: 5 to 15 percent of rhodopuesdomonas pulstris seed culture solution, 25 to 35 percent of candida utilis seed culture solution, 30 to 40 percent of enterococcus faecalis seed culture solution, 10 to 20 percent of lactobacillus acidophilus seed culture solution, 4 to 6 percent of bacillus subtilis seed culture solution and 4 to 6 percent of bacillus licheniformis seed culture solution. The microbial compound preparation disclosed by the invention has the advantages that water quality can be improved, water quality stability is maintained, a bait coefficient is reduced, the intestinal environment is regulated, intestinal bacteria are inhibited, the quality of aquatic products is improved and the output is increased.

Description

technical field [0001] The invention relates to a microbial compound preparation, in particular to a microbial compound preparation for fish and shrimp and a preparation method thereof. Background technique [0002] In recent years, aquaculture animal diseases, especially prawn diseases, have seriously affected the sustainable development of aquaculture, causing significant economic losses, and increasing year by year. The blind use of chemical drugs and fungicides, especially antibiotics, has caused ecological deterioration of the aquaculture water environment and coastal environmental pollution. Drug residues in aquatic animals also have adverse effects on public health. It has become a consensus in aquaculture to formulate a new healthy aquaculture program from the perspective of ecological balance, and the use of probiotics is one of the important measures. [0003] The intestinal tract of aquaculture animals is not only the site of feed digestion and nutrient absorpti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34A23K50/80A23K10/18C12R1/72C12R1/23C12R1/125C12R1/10C12R1/01C02F103/20
Inventor 宋华文王金才于鸽王国华
Owner ZHANJIANG EVERGREEN CULTURE TECH SERVICE
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