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A fertilization hatching method for sticky egg-laying fish

A technology for sticky eggs and fish, applied in the field of aquaculture, can solve the problems of high probability of contamination, decreased hatching rate, low fertilization hatching rate, etc., achieve uniform distribution, avoid fertilization, prolong fertilization time, and ensure the effect of sufficient fertilization

Active Publication Date: 2020-09-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are quite a few fishes that produce sticky eggs. Due to their sticky characteristics, there are many problems in the fertilization process of sticky eggs, such as fertilized eggs are easy to agglomerate, resulting in a decrease in hatchability, and the uniformity of the debonding agent is unstable when using a debonding agent. Incomplete manual debonding, high probability of contamination and other problems will lead to low fertilization and hatching rate, so that the number of fry obtained in aquaculture is small, which greatly restricts the economic benefits of farmers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Fertilization and hatching method of catfish eggs

[0041] Mix the fertilized eggs frozen in the preservation solution at a quantitative ratio of 3:1, then quickly add the prepared NaCl solution with a mass concentration of 5‰, stir evenly for 1 min, and spread the fertilized eggs on the pre-prepared clean water On a 25-mesh polyethylene mesh, clean water at 23°C, arrange the fertilized eggs evenly within 3 minutes, slowly take them out of the water, expose to the air for 5 seconds, then slowly immerse in water for 5 seconds, repeat 3 times, the fertilized eggs are firmly attached to the mesh, and put The mesh is transferred to the hatching tank, and the air is introduced to make the dissolved oxygen amount to 8mg / L. Keep the hatching tank continuously inflated and change the water, and the hatching can be hatched under normal hatching conditions. The above fertilization and incubation method was repeated to set 3 repetitions. Statistical fertilization rate and hatchin...

Embodiment 2

[0044] Fertilization and hatching methods of crucian carp, bream and yellow catfish

[0045] Mix the sperm and eggs of crucian carp or bream frozen in the preservation solution at a ratio of 3:1, and the sperm and eggs of yellow catfish at a ratio of 5:1, and then quickly add them in advance The prepared NaCl solution with a mass concentration of 5‰, after uniform stirring for 1min, spread the fertilized eggs on a 40-mesh polyethylene mesh, place the mesh in a NaCl solution with a mass concentration of 3‰, and clean water at 23°C, spread the fertilized eggs evenly When finished, slowly take out the NaCl solution and transfer it to clean water. At this time, the fertilized eggs will quickly produce stickiness and attach to the mesh, and then transfer the mesh to the hatching tank. The hatching method is the same as that of the largemouth catfish.

[0046] According to statistics, the fertilization rate of crucian carp, bream and yellow catfish is 82.5%±4.6; the hatching rate is...

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Abstract

The invention provides a method for fertilizing and hatching fishes capable of laying adhesive eggs, and belongs to the technical field of aquaculture. The method for fertilizing and hatching the fishes capable of laying the adhesive eggs comprises the following steps: mixing and fertilizing sperm and eggs of fishes capable of laying the adhesive eggs in a NaCl solution with the mass concentrationof 4%-6% to obtain fertilized eggs; placing a net sheet in a water body in advance, uniformly spreading the fertilized eggs on the net sheet in the water body, wherein the spreading time is controlled within 3 min; transferring the net sheet on which the fertilized eggs are spread from the water body to a viscous environment to enable the fertilized eggs to be viscous and then placing the fertilized eggs in the water body to obtain fertilized eggs attached to the net sheet; and incubating the fertilized eggs attached to the net sheet. The method is simple and efficient, and the fertilizationrate and the hatching rate of the adhesive eggs can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for fertilizing and hatching fish that produce viscous eggs. Background technique [0002] In the work of artificial insemination, the hatching of fertilized eggs has always been a very important link. The method of hatching in the outdoor aphrodisiac is not stable, and it is greatly affected by the weather and the outdoor environment. There are quite a few fishes that produce sticky eggs. Due to their sticky characteristics, there are many problems in the fertilization process of sticky eggs, such as fertilized eggs are easy to agglomerate, resulting in a decrease in hatchability, and the uniformity of the debonding agent is unstable when using a debonding agent. Incomplete manual debonding, high probability of contamination and other problems will lead to low fertilization hatching rate, so that the number of fry obtained during aquaculture is small, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/17
CPCY02A40/81
Inventor 汤蓉姚朝瑞彭智李大鹏
Owner HUAZHONG AGRI UNIV
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