Chromatography-mass spectrometry detection method for glucocorticoids and sex hormones in cosmetics

A glucocorticoid and mass spectrometry detection technology, which is applied in the field of pretreatment reagents, can solve the problems of easily polluting the chromatographic column, poor purification effect, complicated treatment process, etc., so as to avoid damage to the chromatographic column and contamination of the instrument, and the purification effect is good. good repeatability

Active Publication Date: 2021-11-19
NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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Problems solved by technology

The pre-processing process is complicated, time-consuming and labor-intensive, and the efficiency is low
In the 2015 version of the Cosmetics Safety and Technical Specifications, seven hormones including progesterone were extracted by methanol, filtered and injected directly, the purification effect is too poor, and it is easy to contaminate the chromatographic column

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  • Chromatography-mass spectrometry detection method for glucocorticoids and sex hormones in cosmetics
  • Chromatography-mass spectrometry detection method for glucocorticoids and sex hormones in cosmetics
  • Chromatography-mass spectrometry detection method for glucocorticoids and sex hormones in cosmetics

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Embodiment Construction

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0034] 1. Specific examples

[0035] A pretreatment system for the detection of glucocorticoids and sex hormones in cosmetics, including a special extraction agent and a special purification tube, the special extraction agent is composed of acetonitrile, methanol, tetrahydrofuran, dimethyl sulfoxide and water in a volume ratio of 3:4:2:1 : 3 ratio mixed; the special purification tube is in a 15mL test tube, containing 75mg C 4 Filler (silica gel bonded butyl group filler, particle size 40-50μm) and 4 ceramic homogeneous particles, the size of the ceramic homogeneous particle is 3mm in diameter x 6mm in length.

[0036] The above-mentioned pretreatment reagent is used for the pretreatment method of the chromatographic-mass spectrometry detection of glucocorticoids and sex hormones in cosmetics, comprising the following steps:

[0037] (1) Ext...

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Abstract

The invention discloses a pretreatment system for detection of glucocorticoids and sex hormones in cosmetics, and a pretreatment method and application for chromatographic-mass spectrometry detection. The invention is characterized in that it includes a special extraction agent and a special purification tube. Dimethyl sulfoxide and water are mixed in a volume ratio of 3:4:2:1:3; the special purification tube contains C 4 Filler and several grains of ceramic homogeneous, C 4 The filling amount of the filler is 50 mg / mL solvent; its pretreatment method for chromatographic-mass spectrometry detection includes the following steps: 1) After weighing the cosmetic sample to be tested into a centrifuge tube, add special extractant and n-hexane, vortex Mix and centrifuge into two layers; 2) Transfer the lower supernatant to a special purification tube and vortex to mix thoroughly. Shake quickly and then centrifuge. Dilute the supernatant and filter it through a filter into a sample bottle for LC-MS / MS determination. , the advantages are good purification effect, ideal recovery rate and precision, and at the same time fast and easy.

Description

technical field [0001] The invention relates to a pretreatment reagent, in particular to a pretreatment method and application for chromatographic-mass spectrometry detection of glucocorticoids and sex hormones in cosmetics. Background technique [0002] The matrix of cosmetics is extremely complex, and its oil content is high, especially lotion, hand cream, shampoo, etc. Low levels of glucocorticoids and sex hormones are typically analyzed by LC-MS / MS. Glucocorticoids and sex hormones are fat-soluble compounds, and their extraction is likely to bring fat-soluble impurities, causing serious interference. Therefore, degreasing treatment is indispensable. Hexane degreasing is the easiest and most commonly used degreasing method. Generally, 50% to 80% of acetonitrile or methanol aqueous solution is used to dissolve, and then washed with n-hexane to remove fat. However, under this condition, glucocorticoids and sex hormones are easy to lose, especially glucocorticoids contain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/14G01N30/02
CPCG01N30/02G01N30/06G01N30/14
Inventor 湛嘉倪梅林徐旭文王春芳周虹玲曹国洲陈先锋
Owner NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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