Tissue culture and rapid propagation method of anemones rivularis

A technology for tissue culture and rapid propagation of saliva, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of slow multiplication, difficulty in meeting the urgent needs of saliva, and easy infection of seedlings by diseases and insect pests To achieve the effect of increasing the amount of seedlings used, expanding the number of germplasm resources, and increasing the reproduction speed

Active Publication Date: 2019-09-13
CHUXIONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Branch propagation can maintain the original species characteristics without variation, but the seedlings are easily infected by diseases and insect pests during the propagation process, and the propagation speed is slow, so it is difficult to meet the urgent demand of the market for the production of Sagittarius

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) on the ultra-clean workbench, cut the young leaves of Sagittarius as explants, and sterilize the surface of the explants with 75% alcohol and 5% sodium hypochlorite solution;

[0040] 2) The explants after surface disinfection were cross-cut into 2mm squares along the vertical midrib direction, and the leaf backs of the explants were inoculated in the induction medium of culture bottles, and 10 plants of the explants were inoculated in each bottle. said explant;

[0041] 3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then cultured for 20 days under the conditions of light intensity of 800Lux, light time of 12h / d, and temperature of 20°C to obtain induced adventitious buds;

[0042] 4) Inoculate the induced adventitious buds into the rooting medium, and the number of inoculations in each bottle of rooting medium is 10, and the light intensity is 800Lux, the light time is 12h / d, and the temperature is 20°C. Take...

Embodiment 2

[0046] 1) on the ultra-clean workbench, cut the young leaves of Sagittarius as explants, and sterilize the surface of the explants with 75% alcohol and 5% sodium hypochlorite solution;

[0047] 2) The explants after surface disinfection were cross-cut into 6mm squares along the vertical midrib direction, and the leaves of the explants were inoculated in the induction medium of the culture bottle downwards, and 10 plants of the explants were inoculated in each bottle. said explant;

[0048] 3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then cultured for 35 days under the conditions of light intensity of 2000Lux, light time of 14h / d, and temperature of 28°C to obtain induced adventitious buds;

[0049] 4) The induced adventitious buds were inoculated into the rooting medium, and the number of inoculations in each bottle of rooting medium was 10, and the light intensity was 2000Lux, the light time was 14h / d, and the temper...

Embodiment 3

[0053] 1) on the ultra-clean workbench, cut the young leaves of Sagittarius as explants, and sterilize the surface of the explants with 75% alcohol and 5% sodium hypochlorite solution;

[0054] 2) The explants after surface disinfection were cross-cut into 4mm squares along the vertical midrib direction, and the leaf backs of the explants were inoculated in the induction medium of the culture bottle, and 10 plants of the explants were inoculated in each bottle. said explant;

[0055] 3) The explants inoculated in the culture bottle were first subjected to dark treatment for 3 days, and then cultured for 30 days under the conditions of light intensity of 1500ux, light time of 12h / d, and temperature of 25°C to obtain induced adventitious buds;

[0056] 4) Inoculate the induced adventitious buds into the rooting medium, and the number of inoculations in each bottle of rooting medium is 10 strains, and the light intensity is 1500Lux, the light time is 12h / d, and the temperature is 2...

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Abstract

The invention discloses a tissue culture and rapid propagation method of anemones rivularis. The method comprises the steps as follows: 1) disinfection is performed; 2) explants after surface disinfection are transversely cut into cubes of 2-6 mm in the direction perpendicular to midribs, an induction medium of a culture flask is inoculated with the explants with blade back facing downwards, and each flask is inoculated with 10 explants; 3) induction of adventitious buds is performed; 4) a rooting medium is inoculated with the induced adventitious buds, and the adventitious buds can take rootsafter being cultured for 60-70 days. An anemones rivularis tissue culture and rapid propagation system is established by in-vitro culture of the anemones rivularis, the survival rate can reach 95% orabove, and the tissue culture and rapid propagation method of anemones rivularis solves the problems of rare anemones rivularis seeds, low germination rate and low propagation speed and is applicableto industrial seedling culture of the anemones rivularis; the breeding speed is obviously increased, so that the use quantity of rare Yi medicine anemones rivularis seedlings is increased, the numberof germplasm resources of the anemones rivularis is increased, meanwhile, the cost can be reduced, and the method is simple to operate and high in economic benefit.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine planting, in particular to a method for tissue culture and rapid propagation of Sagittarius sativa. Background technique [0002] Anemones rivularis Buch.-ham.ex Dc, also known as Caoyumei, Jianfengqing, Jianfenglan, is a perennial herb medicinal plant of the family Ranunculaceae. , anti-cancer effects. Sagittarius often grows in mountains, grasslands, streamsides or other wetlands or forest edges in Yunnan at an altitude of 1750m to 3300m. Secondly, it is also distributed in Sichuan, Guizhou, southwestern Gansu, southeastern Qinghai, southwestern Hubei, western Guangxi, southern and eastern Tibet. However, with the change of environmental conditions, the wild Sagittarius is on the verge of extinction, resulting in a shortage of resources, and the supply exceeds demand. . [0003] Under natural conditions, the seed-setting rate of Sagittarius is very low, and the seed life ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李国树王振吉王浩华徐成东
Owner CHUXIONG NORMAL UNIV
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