Qualitative and quantitative determining method for analyzed object

Abstract

The invention provides a qualitative and quantitative determining method for analyzed object which comprises steps of sample feeding, incubation, transfer, cleaning and detection. Incubation and cleaning are carried out in an incubation module and a cleaning module respectively, and a group of solid-phase units in different reaction phases is incubated in the incubation module simultaneously with the same start and stop time and cleaned in the cleaning module simultaneously with the same start and stop time. Based on that the analyzed object needs incubation and cleaning in each reaction phase, the solid-phase units are incubated and cleaned simultaneously by means of automatic transfer. According to the determining method, samples are detected sequentially under the same processing condition, and a detection result is more accurate; the solid phase units in different phases can be incubated and cleaned simultaneously, and the detection efficiency is improved, and the method can be used to realize instant detection, and is suitable for application scenes as hospital clinics, emergency and physical examination centers.

Description

Technical field [0001] The present invention relates to the technical field of immunoassays, in particular to a method for qualitative and quantitative determination of an analyte. Background technique [0002] The phenomenon of specific and spontaneous binding of specific ligands, such as immunoassay for antigen-antibody specific reaction and nucleic acid analysis for complementary nucleic acid base pairing, is widely used in many fields. POCT (point-of-care testing), point-of-care testing refers to a testing method that is carried out at the sampling site and uses portable analytical instruments and supporting reagents to quickly obtain test results. However, the existing analyte determination methods are due to The analysis system is equipped with sample bins, reaction cup storage devices, and sample addition devices, resulting in a complex structure, large volume, and inportability of the entire analysis system. On the other hand, although the existing measurement methods can...

AI Technical Summary

Problems solved by technology

POCT (point-of-care testing), real-time detection refers to a detection method that is carried out at the sampling site and uses portable analytical instruments and supporting reagents to quickly obtain test results. However, the existing analyte determination methods are due to The analysis system is equipped with a sample chamber, a cuvette storage device, and a sample adding device, etc., resulting in a complex structure of the entire analysis system, a large volume, and not portable; It can detect one indicator, but cannot complete the test of multiple indicators at the same time

In this way, if multiple indicators are detected, steps such as sample addition, incubation, cleaning, and detection are required for each detection, and the detection time is long and the detection efficiency is low.

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