Pleurotus nebrodensis wild in-situ conservation method

The technology of Bailing mushroom and Bailing mushroom is applied in the fields of botanical equipment and methods, mushroom cultivation, horticulture, etc., and can solve the problems of low preparation efficiency of liquid strains of Asafoetida, easy inactivation of liquid strains, etc. Conducive to field conservation, wide applicability, and the effect of improving colonization efficiency

Active Publication Date: 2019-09-27
INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems in the prior art that the preparation efficiency of liquid strains of Ferulicum ferulae is low, and the liquid strains prepared by the method of inoculating Ferula ferulae plants in the field with Ferula ferulae liquid strains are easy to inactivate the liquid strains in the process of inoculating Ferula ferulae plants in the field, etc. , there is no better technical status about the in-situ conservation of Bailing mushroom germplasm resources in the field. The present invention aims to provide a method for in-situ conservation of Bailing mushroom in the field. By preparing the homogenate of Bailing mushroom fruiting bodies, the Bailing The homogenate of mushroom fruiting bodies was inoculated in ferulus plants in the field, and the technology of inoculating ferulic plants in the field with homogenous fruiting bodies of Bailing mushroom was established, and the colonization of Bailing mushroom mycelium was realized. This method is simple and fast, and is suitable for large-scale field cultivation Large-scale inoculation of Ferulicum plants overcomes the objective shortcomings of existing technologies, can effectively improve the colonization efficiency of Bailing mushroom mycelium in the roots of wild Ferulic plants, and has made important achievements in the in-situ conservation technology of Bailing mushroom germplasm resources. Progress, so that the germplasm resources of Bailing mushroom can be protected and developed, and it has wide applicability in the protection of germplasm resources of Bailing mushroom

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pleurotus nebrodensis wild in-situ conservation method
  • Pleurotus nebrodensis wild in-situ conservation method
  • Pleurotus nebrodensis wild in-situ conservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Selection of the fruiting body of Bailing mushroom, the artificially cultivated Bailing mushroom fruiting body is selected. In order to ensure the vitality of the fruiting body to germinate into mycelium, the fruiting body should be fresh, no mildew, and no disease and insect infection. The fruiting body of Bailing mushroom is better;

[0025] (2) Preparation of the fruiting body homogenate of Bailing mushroom, the fruiting body of Bailing mushroom prepared in step (1) is cut into fungus blocks, put into a tissue crusher, and the same volume of sterile water is added, and the fruiting body is broken into pieces Homogenize, pour it into a container, and add 1 / 3 to 1 / 2 volume of sterile water to dilute to obtain a homogenate of Bailing mushroom fruiting body, which is ready for use;

[0026] (3) asafoetida plant selection, observe the asafoetida plant and its rhizomes, select the forefusalem plant without bolting, the 2nd-3rd year, and the root without the asafoetida ...

Embodiment 2

[0033] (1) Selection of the fruiting body of Bailing mushroom, the artificially cultivated Bailing mushroom fruiting body is selected. In order to ensure the vitality of the fruiting body to germinate into mycelium, the fruiting body should be fresh, no mildew, and no disease and insect infection. The fruiting body of Bailing mushroom is better;

[0034](2) Preparation of the fruiting body homogenate of Bailing mushroom, the fruiting body of Bailing mushroom prepared in step (1) is cut into fungus blocks, put into a tissue crusher, and the same volume of sterile water is added, and the fruiting body is broken into pieces Homogenize, pour it into a container, and add 1 / 2 volume of sterile water to dilute to obtain a homogenate of the fruiting body of Bailing mushroom, which is ready for use;

[0035] (3) asafoetida plant selection, observe the asafoetida plant and its rhizomes, select no bolting, the second year of life, and the root part does not have the asafoetida plant of B...

Embodiment 3

[0041] To explore the four factors that affect the colonization rate of Bailing mushroom (Y): dilution with water after the homogenization of Bailing mushroom (A), the amount of asafoetida plants inoculated with the fruiting body of Bailing mushroom (B), and the growth time of asafoetida plants (C). ), a four-factor and three-level orthogonal experiment was performed, and the factors and levels of the orthogonal experiment are shown in Table 1.

[0042] Table 1: Orthogonal test factor and level table

[0043]

[0044] Table 2 shows the results and analysis of the orthogonal optimization of the colonization rate of Bailing mushroom.

[0045] Table 2: Orthogonal test results and analysis

[0046]

[0047] As can be seen from Table 2, within the scope of the experimental design, the dilution with water after homogenization of Bailing mushroom has the greatest influence on the colonization rate of Bailing mushroom. Combined with Table 1 and Table 2, the optimal optimizatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a pleurotus nebrodensis wild in-situ conservation method. A pleurotus nebrodensis sporocarp homogenate is prepared and is inoculated to a Ferulic plant in the wild, the technology of inoculating the pleurotus nebrodensis sporocarp homogenate to the Ferulic plant in the wild is established, the average successful planting rate of pleurotus nebrodensis mycelia reaches 83.02%, the planting of the pleurotus nebrodensis mycelia is realized, the method is simple, rapid and suitable for inoculating pleurotus nebrodensis to the Ferulic plant on a large scale in the wild, the efficiency of planting the pleurotus nebrodensis mycelia on the root of the Ferulic plant in the wild can be effectively improved, an important progress is made on the pleurotus nebrodensis germplasm resource in-situ conservation technology, the pleurotus nebrodensis germplasm resources are protected and developed, and the method has wide applicability on the aspect of the protection of the pleurotus nebrodensis germplasm resources.

Description

technical field [0001] The invention relates to the technical field of field conservation of edible fungus germplasm resources, in particular, to the technical field of a method for field conservation of Bailing mushroom in the field. Background technique [0002] Bailing Mushroom, the Chinese name is Pleurotus tuoliensis, is a rare and delicious edible mushroom with high food and medicinal value. The article "The famous cultivated mushroomBailinggu is a separate species of the Pleurotus eryngii species complex" published by Mengran Zhao et al. in 2016, and the article "New Notes on the Scientific Names of Five Important Edible Mushrooms in China" published by Dai Yucheng et al. in the Journal of Mycology in 2018 In , the two fungi of Pleurotus ferrugifolia and Pleurotus bailing were distinguished by phylogenetic research, they were two independent branches in phylogeny, and the support rate was very high, in addition, the distribution areas of the two species were also diff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/40
CPCA01G18/00A01G18/40
Inventor 贾培松罗影贾文捷魏鹏管建华努尔孜亚·亚力买买提丁丽丽郝敬喆高加强赛力克·阿克孜木别克王明智
Owner INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap