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A method for in situ conservation of Bailing mushroom in the wild

A technology of Bailing mushroom and Bailing mushroom, which is applied in the fields of botany equipment and methods, mushroom cultivation, gardening, etc., can solve the problems of low preparation efficiency of liquid strains of Ferula ferulae and easy inactivation of liquid strains, and achieve Conducive to field conservation, wide applicability, and the effect of improving colonization efficiency

Active Publication Date: 2022-05-10
INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems in the prior art that the preparation efficiency of liquid strains of Ferulicum ferulae is low, and the liquid strains prepared by the method of inoculating Ferula ferulae plants in the field with Ferula ferulae liquid strains are easy to inactivate the liquid strains in the process of inoculating Ferula ferulae plants in the field, etc. , there is no better technical status about the in-situ conservation of Bailing mushroom germplasm resources in the field. The present invention aims to provide a method for in-situ conservation of Bailing mushroom in the field. By preparing the homogenate of Bailing mushroom fruiting bodies, the Bailing The homogenate of mushroom fruiting bodies was inoculated in ferulus plants in the field, and the technology of inoculating ferulic plants in the field with homogenous fruiting bodies of Bailing mushroom was established, and the colonization of Bailing mushroom mycelium was realized. This method is simple and fast, and is suitable for large-scale field cultivation Large-scale inoculation of Ferulicum plants overcomes the objective shortcomings of existing technologies, can effectively improve the colonization efficiency of Bailing mushroom mycelium in the roots of wild Ferulic plants, and has made important achievements in the in-situ conservation technology of Bailing mushroom germplasm resources. Progress, so that the germplasm resources of Bailing mushroom can be protected and developed, and it has wide applicability in the protection of germplasm resources of Bailing mushroom

Method used

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  • A method for in situ conservation of Bailing mushroom in the wild
  • A method for in situ conservation of Bailing mushroom in the wild
  • A method for in situ conservation of Bailing mushroom in the wild

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Bailing mushroom fruiting body selection, select the artificially cultivated Bailing mushroom fruiting body, in order to ensure the vitality of the fruiting body to germinate into mycelium again, the fruiting body should be fresh, no mildew, no disease and insect infection, and freshly picked The fruiting body of Bailing mushroom is better;

[0025] (2) Homogenate preparation of the fruiting body of Bailing mushroom, cut the fruiting body of Bailing mushroom prepared in step (1) into bacterial pieces, put it into a tissue crusher, add the same volume of sterile water, and break the fruiting body into Homogenize, pour into a container, then add 1 / 3 to 1 / 2 volume of sterile water to dilute to obtain a homogenate of Bailing mushroom fruiting body, set aside;

[0026] (3) Asafoetida plants are selected, observe the asafoetida plants and their rhizomes, select no bolting, the 2-3 year is born, and the roots do not have the ferulae plants colonized by the mycelium of Bail...

Embodiment 2

[0033] (1) Bailing mushroom fruiting body selection, select the artificially cultivated Bailing mushroom fruiting body, in order to ensure the vitality of the fruiting body to germinate into mycelium again, the fruiting body should be fresh, no mildew, no disease and insect infection, and freshly picked The fruiting body of Bailing mushroom is better;

[0034](2) Homogenate preparation of the fruiting body of Bailing mushroom, cut the fruiting body of Bailing mushroom prepared in step (1) into bacterial pieces, put it into a tissue crusher, add the same volume of sterile water, and break the fruiting body into Homogenate, pour it into a container, and then add 1 / 2 volume of sterile water to dilute to obtain a homogenate of Bailing mushroom fruiting bodies, and set aside;

[0035] (3) Asafoetida plant selection, observe the Ferulae plant and its rhizome, select no bolting, live in the second year, and the root does not have the Ferula plant colonized by Bailing mushroom myceliu...

Embodiment 3

[0041] Explore the four factors that affect the colonization rate of Bailing mushroom (Y): the homogenate of Bailing mushroom was diluted with water (A), the amount of ferulae plant inoculated with the homogenate of Bailing mushroom fruiting body (B), and the growth time of ferulae plant (C ), carry out four-factor three-level orthogonal experiment, the factors and levels of orthogonal experiment are shown in Table 1.

[0042] Table 1: Orthogonal test factor and level table

[0043]

[0044] See Table 2 for the test results and analysis of the orthogonal optimization of Bailing mushroom colonization rate.

[0045] Table 2: Orthogonal test results and analysis

[0046]

[0047] It can be seen from Table 2 that, within the scope of the experimental design, dilution with water after homogenization of Bailing mushroom has the greatest impact on the colonization rate of Bailing mushroom. Combining Table 1 and Table 2 to get the best optimization condition is A 1 B 2 C 2 ...

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Abstract

The invention discloses a method for in-situ conservation of Bailing mushroom in the field. By preparing the homogenate of Bailing mushroom fruiting body, inoculating the Bailing mushroom fruiting body homogenate in the field to ferulae plants, and establishing the Bailing mushroom fruiting body homogenate in the field The technology of inoculating ferulic plants, the average successful colonization rate of Bailing mushroom mycelium is 83.02%, realizes the colonization of Bailing mushroom mycelium, and this method is simple and fast, suitable for large-scale inoculation of ferulic plants in the field, can Effectively improve the colonization efficiency of Bailing mushroom mycelium in the roots of wild ferulae plants, and make important progress in the in-situ conservation technology of Bailing mushroom germplasm resources, so that Bailing mushroom germplasm resources can be protected and developed. It has wide applicability in the protection of mushroom germplasm resources.

Description

technical field [0001] The invention relates to the technical field of field conservation of edible fungus germplasm resources, in particular, the invention relates to the technical field of a method for field conservation of Bailing mushroom. Background technique [0002] Bailing mushroom, whose Chinese name is Pleurotus tuoliensis, is a rare and delicious edible fungus with high food and medicinal value. In 2016, the article "The famous cultivated mushroomBailinggu is a separate species of the Pleurotus eryngii species complex" published by MengranZhao et al. and in 2018 in the "Acta Mycology" by Dai Yucheng et al. In , through phylogenetic research, the two fungi of Pleurotus ferulae and Pleurotus bailing were distinguished. They are two independent branches in phylogeny, and the support rate is very high. In addition, the distribution areas of the two species are also different. , so Bai Ling Xian Er was promoted to an independent species. In my country, wild Bailing m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/40
CPCA01G18/00A01G18/40
Inventor 贾培松罗影贾文捷魏鹏管建华努尔孜亚·亚力买买提丁丽丽郝敬喆高加强赛力克·阿克孜木别克王明智
Owner INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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