Plant extract for inhibiting tyrosinase activity and its application

A plant extract and tyrosinase technology, applied in the field of cosmetics preparation, can solve the problems of low activity, unclear active small molecules, and many side reactions

Active Publication Date: 2022-03-22
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has been found that a variety of plant extracts contain substances that inhibit tyrosinase activity, such as fairy honey fruit, chickweed, black leek, weeping grass, humulus aerial parts, iroko mulberry, gall gall, and thistle whole Grass, black rice bran, and active substances that have been proven to be effective include arbutin, glutathione, chitooligosaccharides, oxidized resveratrol, etc. In addition to the significant curative effect of arbutin, several other known active molecule effects Poor, while industrial production of arbutin has a low yield, many side reactions, and produces chemical substances that are harmful to the human body
[0004] In vivo, small molecule metabolites that inhibit tyrosinase activity are extremely complex and often have multiple conformations. Small molecules with different conformations have different activities. The active small molecules contained in most plant extracts are not clear and have low activity. Combining several existing plant extracts often causes mutual antagonism and lower activity. It is a research in this field to find a plant extract with high activity of inhibiting tyrosinase or several plant extracts that are synergistic. difficulty

Method used

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  • Plant extract for inhibiting tyrosinase activity and its application
  • Plant extract for inhibiting tyrosinase activity and its application
  • Plant extract for inhibiting tyrosinase activity and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Effect of Plant Extracts on Melanoma Cell Proliferation

[0036] Dilute each plant extract sample with liquid medium to a concentration of 6.25, 12.5, 25, 50, and 100 μg / mL, and filter it with a 0.20 μm sterile filter head before use.

[0037] B16 mouse melanoma cells in the logarithmic growth phase were selected, digested with 0.25% trypsin, and prepared with RPMI-1640 complete medium at a density of 1×10 5 The cell suspension of each was seeded in a 96-well culture plate, 100 μL per well, at 37 °C, 5% CO 2 Cultured in an incubator. After adhering to the wall, add complete culture medium containing different concentrations of samples, add an equal volume of cell suspension to the control group, and add an equal volume of complete medium to the blank control group, 100 μL per well. Set up 3 replicate wells for each concentration, 37°C, 5% CO 2 After culturing in the incubator for 72h, the absorbance (OD) value was measured at 570nm in a microplate reader by...

Embodiment 2

[0044] Example 2 Effect of Plant Extracts on Melanin Synthesis in Melanoma Cells

[0045] The 3rd passage melanoma cells were cultured at 1×10 4 Each density was connected to a 6-well plate, and the medium was changed after 24 hours. Add the test sample to 3 wells of each drug, and the sample concentration is the same as in Example 1. In the control group, RPMI1640 culture fluid was used instead of the sample solution. 37°C, 5% CO 2 After 72 h of incubation, the supernatant was discarded. Add 2.5g / L trypsin to each well and digest at room temperature for 5min. Add 4mL of culture medium to stop the digestion, and pipette into a single cell suspension. Take 0.5mL for cell counting, centrifuge the rest of the cell suspension at 1500r / min for 10min, discard the supernatant, add 1mL of 1mol / L NaOH, incubate in a water bath at 90°C for 2h, and measure the absorbance at 490nm with a microplate reader. The amount of melanin synthesis and the inhibition rate of drugs on melanin s...

Embodiment 3

[0056] Face cream, its preparation method is as follows:

[0057] (1) Accurately weigh 3 g of ginkgo extract and 3 g of pomegranate peel extract, and put them into a beaker;

[0058] (2) Measure 100ml of distilled water and pour it into a beaker, put it into a magnet, and heat and dissolve in a magnetic stirrer for 1.5h;

[0059] (3) After the heating and dissolving is completed, first filter once, then filter once to obtain the clarified liquid of the plant extract;

[0060] (4) Take another clean beaker, measure 20ml of the plant extract clarified liquid with a graduated cylinder and pour it into the beaker, then add 200ml of distilled water and stir;

[0061] (5) Weigh 4g of emulsifier, 2g of glycerin, measure 10ml of dipropylene glycol and pour them into a beaker together, and stir for 5min;

[0062] (6) Add 100uL PCA-Na and 10uL essence dropwise, and stir for 5min;

[0063] (7) Packaging.

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Abstract

The invention discloses a plant extract for inhibiting tyrosinase activity and application thereof. The plant extracts are ginkgo biloba extract, pomegranate bark extract, pyracantha extract, angelica dahurica extract, and bindflower extract. The plant extracts prepared by the present invention have the effect of inhibiting the synthesis of melanin in melanoma cells. Among them, it is found that Ginkgo biloba extract, pomegranate peel extract, Pyracantha japonicus extract and Convolvulaceae extract are used in combination, which has obvious effect. The synergistic effect can be used as the active material raw material for the preparation of whitening cosmetics.

Description

technical field [0001] The invention belongs to the technical field of cosmetic preparation, and in particular relates to a plant extract for inhibiting tyrosinase activity and application thereof. Background technique [0002] The color of human skin depends on the content and distribution of melanin. The physiological process of melanin formation can be basically summarized into three stages: melanin cells produce melanin; melanin granules transfer to keratinocytes through melanocyte dendrites; Melanin granules ascend to the stratum corneum along with the epidermal cells, and are excreted as the stratum corneum falls off. [0003] Tyrosinase is the key enzyme for the synthesis of melanin in organisms. The main function of commonly used whitening cosmetics is to affect the metabolism of tyrosinase, melanocytes and melanin. At present, it has been found that a variety of plant extracts contain substances that inhibit tyrosinase activity, such as fairy honey fruit, chickweed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K8/9771A61Q19/02
CPCA61K8/9789A61K8/9771A61Q19/02A61K2800/5922A61K2800/782
Inventor 陈小明李湘玉贺靖刘艳芳蒋丽林何福林吕敬崑
Owner HUNAN UNIV OF SCI & ENG
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