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Kit for early warning of colorectal cancer metastasis

A colorectal cancer and kit technology, which is applied in the medical and health field, can solve the problems of inappropriate screening and census, great injury and pain of patients, and high technical requirements of operators

Inactive Publication Date: 2019-11-19
谢丽
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Problems solved by technology

[0005] The use of colonoscopy can improve the diagnosis rate of colorectal cancer, but it is an interventional examination, which causes great damage and pain to the patient, and has high technical requirements for the operator, and not all parts of the colorectum can be biopsied. Suitable for large-scale population screening censuses

Method used

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  • Kit for early warning of colorectal cancer metastasis
  • Kit for early warning of colorectal cancer metastasis
  • Kit for early warning of colorectal cancer metastasis

Examples

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Embodiment example 1

[0022] Implementation case 1: application of microRNAs: miR-320d in early warning of CRC metastasis

[0023] 1. Experimental design

[0024] The experiment was designed to collect serum samples from newly diagnosed CRC patients and healthy volunteers in Shandong Cancer Hospital, isolate and enrich exosomes, and obtain exosomal RNA. The expression of miR-320d was detected by qPCR, the difference of its expression between metastatic colorectal cancer and non-metastatic colorectal cancer was statistically analyzed, and its diagnostic efficacy was analyzed.

[0025] , patients and samples involved in the experiment

[0026] A total of 108 patients with non-metastatic CRC and 34 patients with metastatic CRC who were admitted to Shandong Provincial Cancer Hospital Affiliated to Shandong First Medical University from December 2017 to July 2018 were included, and TNM classification was performed based on postoperative pathological examination. The clinical data included in this stud...

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Abstract

The invention relates to development for early warning of a colorectal cancer (CRC) metastasis kit based on exosome microRNA:miR-320d, and belongs to the field of medical treatment and public health.A biomarker for early warning of colorectal cancer metastasis is serum exosome miR-320d which includes a reverse transcription reaction system, a fluorescent quantitative PCR reaction system and an internal reference system. Compared with patients with non-metastatic colorectal cancer, the expression level of the miR-320d in the colorectal cancer metastatic serum exosome is increased significantly, ROC curve analysis shows that AUC is 0.633, the sensitivity is 62.0%, and the specificity is 64.7%.

Description

technical field [0001] The invention relates to the development of an early warning kit for colorectal cancer (CRC) metastasis based on exosome microRNA: miR-320d, which belongs to the field of medical and health care. Background technique [0002] Colorectal cancer (CRC) is the third most common cancer worldwide, with 1.36 million new cases and approximately 700,000 deaths each year. About 50% of colorectal cancer patients die from advanced disease (local or distant metastasis). Therefore, there is an urgent need for a more sensitive marker for early warning of metastasis to provide an objective basis for early warning of colorectal cancer metastasis. [0003] Tumor cell-derived exosomes (Tomor-derived exosomes, TDEs) have been shown to promote cancer progression, and the intercellular interactions mediated by exosomes may play an important role in tumorigenesis. Isolation of exosomes and their use in the clinic may be an alternative to invasive surgery to diagnose CRC wh...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 谢丽宋现让
Owner 谢丽
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