A method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice

A technology of fruit juice and sugar alcohol, which is applied in the field of analytical chemistry, can solve the problems of not meeting the detection requirements and the small number of components, and achieve the effects of reducing detection costs, improving accuracy, and improving detection efficiency

Active Publication Date: 2021-06-11
日照海关综合技术服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, although people have studied the methods for the simultaneous detection of multiple sugars, sugar alcohols and alcohols in fruit juice, what can be retrieved in the current database is only for a certain type of components (such as simple sugars or Alcohols) or the number of simultaneously detected components is small (only about 10 kinds of sugars and sugar alcohols are analyzed simultaneously), which cannot meet the growing detection needs. Therefore, research and establish a method with strong applicability, good selectivity, A method for simultaneous detection of multiple sugars, sugar alcohols and alcohols with high sensitivity has important practical significance and application value

Method used

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  • A method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice
  • A method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice
  • A method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice

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Experimental program
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Embodiment 1

[0046] Embodiment 1: a method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice

[0047] 1. Instruments and Reagents

[0048]ICS5000+ ion chromatography system is equipped with electrochemical detector and autosampler (ThermoScientific Company of the United States); Milli-Q ultrapure water instrument (Millipore Company of the United States); electronic balance (ML802 / 02 of Mettler Company); experimental water: super Pure water (resistivity 18.2MΩ.cm); sugar, sugar alcohol, alcohol standard or standard reagent (purity>98%); sodium hydroxide solution (50% w / w, Fisher Chemical); hydrochloric acid (premium pure); IC-RP10 column (Agela Technologies).

[0049] 2. Ion Chromatography Conditions

[0050] CarboPac MA1 (4×250 mm) analytical column, CarboPac MA1 (4×50 mm) guard column; working electrode: Gold (Au); reference electrode: AgCl electrode; mobile phase: NaOH eluent (nitrogen protection); flow rate : 0.40 mL / min; column temperature:...

Embodiment 2

[0058] Example 2: Influence of flow rate on detection

[0059] According to the conditions of the experimental analysis in Example 1, under the condition that other conditions remain unchanged, the flow rate of the eluent is successively tested to be 0.30 mL / min, 0.40 mL / min, 0.50 mL / min, and 0.60 mL / min. The separation of the components of the standard solution, the relationship between the flow rate of the eluent and the system pressure is shown in Table 2.

[0060] Table 2 Relationship between eluent flow rate and system pressure

[0061]

[0062] From Table 2, it can be concluded that when the eluent flow rate is 0.30 mL / min, the system pressure is 885 psi, because the flow rate is lower, the pressure is lower, the peak width increases, the peak sensitivity decreases, and the overall resolution is not high. When the flow rate was 0.50 mL / min, the system pressure reached 1430 psi. Although the component sensitivity was improved, the resolution of individual components w...

Embodiment 3

[0063] Example 3: Influence of multi-stage gradient elution conditions on detection

[0064] According to the conditions of the experimental analysis in Example 1, under the condition of other conditions unchanged, test the initial eluent concentration successively and mix the standard solution components under the conditions of 420, 450, 480, 500, 520, 550 mmol / L respectively Separation situation, since the initial concentration has little effect on the resolution of the three components of components 20, 21, and 22, here we focus on the impact of the initial concentration on the first 19 components, and the component separation of the mixed standard solution is as follows figure 2 shown.

[0065] From figure 2 It can be seen from the figure that when the initial eluting concentrations were 420 and 450 mmol / L, components 7 and 8, and components 10 and 11 were not separated; when the initial eluting concentrations were 480 mmol / L, components 10 and 11 The resolution was no...

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Abstract

The invention discloses a method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice, which belongs to the field of analytical chemistry. The invention adopts integral pulse amperometric-ion chromatography and optimizes the pretreatment method to study the factors that affect the separation of components. Experimental factors, established a one-time simultaneous detection of 22 kinds of sugars, sugar alcohols, alcohols (2,3-butanediol, propylene glycol, methanol, glycerol, erythritol, xylitol, rhamnose , arabitol, sorbitol, trehalose, galactitol, mannitol, 2-deoxy-D-glucose, arabinose, melibiose, glucose, galactose, fructose, ribose, sucrose, raffinose, maltose) The method solves the technical problem of accurate analysis of multiple components of sugars, sugar alcohols and alcohols in fruit juice. This method is fast, accurate and sensitive, and realizes the simultaneous analysis of multiple categories and components. important contribution.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for simultaneously detecting 22 kinds of sugars, sugar alcohols and alcohols in fruit juice. Background technique [0002] Fruit juice is an important beverage in people's daily life, and fruit juice contains protein, amino acid, sugar, sugar alcohol and other ingredients. Among them, carbohydrates are the main source of energy for people to maintain life activities. Sugar alcohol is a substance formed by the reduction of aldehyde or ketone groups of sugars to hydroxyl groups. It is mainly a sweetener produced by the reaction of corresponding sugars. It will not cause blood sugar to rise, so it has the effect of preventing diabetes and obesity. Alcohols are compounds that contain a hydroxyl group bound to a hydrocarbon group or a carbon on the side chain of a benzene ring in the molecule. Many alcohols are beneficial to human health, but some alcohols hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/96G01N30/88G01N30/06G01N30/14G01N30/34G01N30/30
CPCG01N30/06G01N30/14G01N30/30G01N30/34G01N30/88G01N30/96G01N2030/047G01N2030/146G01N2030/884
Inventor 宋卫得高尧华许美玲叶佳宇李林杰林令海
Owner 日照海关综合技术服务中心
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