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A Lactobacillus casei that can regulate the balance of th2/th1 in allergic asthma and its application

A technology of Lactobacillus casei and the number of live bacteria, applied in the field of Lactobacillus casei, can solve the problems of recurrent disease, unclear action relationship, poor compliance, etc., and achieve the effect of reducing lung inflammation

Active Publication Date: 2021-08-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, since most bronchodilator drugs and anti-inflammatory drugs need to be administered by intravenous injection or inhalation, the patient's compliance with these drugs is poor, which leads to repeated attacks of the patient's disease, and over time it will cause the patient's Severe complications such as emphysema and pulmonary heart disease
[0007] Although probiotic preparations are relatively safe and healthy, most of the probiotic preparations on the market may also have the disadvantage of being slow to take effect due to the fact that the currently discovered probiotics that can relieve the symptoms of allergic asthma may have poor or unclear effects. , or have the defect that the role relationship is not clear
[0008] Therefore, there is an urgent need to find a probiotic that is highly specific to allergic asthma and has a strong ability to alleviate allergic asthma to solve the problem of unclear relationship between the effects of existing probiotic preparations for allergic asthma

Method used

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  • A Lactobacillus casei that can regulate the balance of th2/th1 in allergic asthma and its application
  • A Lactobacillus casei that can regulate the balance of th2/th1 in allergic asthma and its application
  • A Lactobacillus casei that can regulate the balance of th2/th1 in allergic asthma and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: Screening and identification of Lactobacillus casei

[0055] 1. Screening:

[0056] Taking infant feces from Wuxi, Jiangsu as the sample, the sample was pretreated and stored in a -80°C refrigerator in about 20% glycerin. After taking it out and thawing, mix the sample and add 0.5mL sample to 4.5mL, then add 0.9 Perform gradient dilution with % normal saline, select the appropriate gradient dilution and spread it on the MRS solid medium, culture at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS solid medium, pick a single colony and transfer to MRS The liquid medium was enriched and preserved in 30% glycerol to obtain strain CCFM1073.

[0057] 2. Identification:

[0058] The 16S rDNA of CCFM1073 was amplified and sequenced, and the sequence was compared in NCBI. The results showed that the strain was Lactobacillus casei, and it was named CCFM1073 as Lactobacillus casei.

Embodiment 2

[0059] Embodiment 2: the cultivation of Lactobacillus casei

[0060] Lactobacillus casei (Lactobacillus casei) CCFM1073 was inserted into MRS solid medium and cultured at 37°C for 48 hours, and its colonies were observed under a microscope. It was found that the colonies were rough, off-white, and sometimes slightly yellow. The shape of the bacteria varies in length, and the two ends are square, often forming chains.

[0061] Insert Lactobacillus casei (Lactobacillus casei) CCFM1073 into MRS liquid medium and culture at 37°C for 18 hours, then transfer to fresh MRS liquid medium, cultivate under the same conditions for 18 hours, centrifuge the bacteria at 6000g for 15 minutes, wash the bacteria with 0.9% normal saline Centrifuge again at 6000 g for 10 min after inoculation to obtain bacterial cells, resuspend with 30% sucrose solution, and freeze at -80°C until use.

Embodiment 3

[0062] Example 3: Effect of Lactobacillus casei on pulmonary inflammation in allergic asthmatic mice

[0063] The 6-week-old SPF grade BALB / c female mice were divided into 6 groups, namely the normal group, the model group and the experimental group. The L.GG prevention group of Bacillus GG (ATCC53103), the L.casei1 prevention group of Lactobacillus casei 1 and the L.casei2 prevention group of Lactobacillus casei 2 by oral administration, 6 animals in each group, were raised in the Experimental Animal Center of Jiangnan University , fed with common feed, constant temperature 21-26°C, humidity 40-70%, noise less than or equal to 60dB, animal illumination 15-20LX (all animal experiment procedures were reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0064] The experiment lasted for 6 weeks (5 days a week), and the model was established in the 2nd to 6th weeks. The mice in the model group and the experimental group were anesth...

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Abstract

The invention discloses a strain of Lactobacillus casei capable of regulating the Th2 / Th1 balance of allergic asthma and an application thereof, belonging to the technical field of microbes and the technical field of medicine. Lactobacillus casei (Lactobacillus casei) of the present invention has the effect of relieving allergic asthma, which is specifically reflected in: (1) significantly reducing the lung inflammatory response of allergic asthma mice; (2) significantly inhibiting the total Production of immunoglobulin IgE; (3) significantly inhibited the production of dust mite-specific immunoglobulin IgG1 in the serum of allergic asthmatic mice; (4) did not change the production of dust mite-specific immunoglobulin IgG2a in serum of allergic asthmatic mice (5) Remarkably restore the balance of Th2 / Th1, therefore, Lactobacillus casei has a huge application prospect in the preparation of products for preventing and / or treating allergic asthma.

Description

technical field [0001] The invention relates to a strain of Lactobacillus casei capable of regulating the Th2 / Th1 balance of allergic asthma and an application thereof, belonging to the technical field of microbes and the technical field of medicine. Background technique [0002] The worldwide prevalence is on the rise. Over the past ten years, there have been about 100 million allergic asthma patients in the world; the prevalence and mortality of allergic asthma in the United States, the United Kingdom, Australia, New Zealand and other countries have gradually increased Trend: There are more than 10 million asthma patients in China, and the average prevalence rate of allergic asthma in the population is about 1%, among which children can reach 3%. In susceptible individuals, this inflammation can cause recurrent episodes of wheezing, shortness of breath, chest tightness, and / or coughing, which can lead to life-threatening complications such as respiratory arrest and respira...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L29/00A23L33/135A61K35/747A61P11/06A61P37/08C12R1/245
CPCA61K35/747A23L29/065A23L33/135A61P11/06A61P37/08C12N1/20C12N1/205C12R2001/245
Inventor 陆文伟陈卫李凌智翟齐啸张秋香王鸿超赵建新张灏
Owner JIANGNAN UNIV
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