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A strain of Lactobacillus reuteri that can relieve allergic asthma and its application

A technology for Lactobacillus reuteri and allergic asthma, applied in the field of Lactobacillus reuteri, can solve the problems of recurrent disease, poor effect, unclear role relationship, etc.

Active Publication Date: 2021-03-02
无锡特殊食品与营养健康研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, since most bronchodilator drugs and anti-inflammatory drugs need to be administered by intravenous injection or inhalation, the patient's compliance with these drugs is poor, which leads to repeated attacks of the patient's disease, and over time it will cause the patient's Severe complications such as emphysema and pulmonary heart disease
[0009] Although probiotic preparations are relatively safe and healthy, the currently discovered probiotics such as Lactobacillus adderiformis, Lactobacillus rhamnosus and Bifidobacterium animalis that have been found to relieve the symptoms of allergic asthma may have poor or unclear effects. Therefore, most probiotic preparations on the market also have the defect of slow effect, or have the defect of unclear action relationship
[0010] Therefore, there is an urgent need to find a probiotic that is highly specific to allergic asthma and has a strong ability to relieve allergic asthma, so as to solve the problems that the existing probiotic preparations for allergic asthma are slow to take effect and the relationship between their effects is not clear

Method used

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  • A strain of Lactobacillus reuteri that can relieve allergic asthma and its application
  • A strain of Lactobacillus reuteri that can relieve allergic asthma and its application
  • A strain of Lactobacillus reuteri that can relieve allergic asthma and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1: Screening and identification of Lactobacillus reuteri

[0061] Specific steps are as follows:

[0062] 1. Screening

[0063] Taking human feces from Dali, Yunnan as samples, the samples were pretreated and stored in a -80°C refrigerator in about 20% glycerin. After taking out and thawing, mix the samples and add 0.5mL samples to 4.5mL to contain 0.05% cysteine ​​and 0.9% normal saline were used for gradient dilution, and the appropriate gradient dilution was selected and spread on the MRS solid medium added with 0.05% cysteine, cultured at 37°C for 48 hours, and typical colonies were picked to Purify by streaking on MRS solid medium, pick a single colony and transfer to MRS liquid medium (containing 0.05% cysteine) for enrichment, and preserve in 30% glycerol to obtain strain CCFM1040, strain R1 and strain R2.

[0064] 2. Identification

[0065] The genome of CCFM1040, R1, R2 was extracted, and the 16S rDNA of CCFM1040, R1, R2 was amplified and sequence...

Embodiment 2

[0066] Embodiment 2: the cultivation of Lactobacillus reuteri

[0067] Specific steps are as follows:

[0068] Lactobacillus reuteri (Lactobacillus reuteri) CCFM1040 was inserted into MRS solid medium (containing 0.05% cysteine) and cultured at 37°C for 48 hours, and its colonies were observed under a microscope, and it was found that The colonies are milky white, irregular, rounded convex, smooth, and the shape of the bacteria is slightly irregular, with rounded ends of Campylobacter, usually in single, paired and small clusters.

[0069] Lactobacillus reuteri (Lactobacillus reuteri) CCFM1040 was inserted into MRS liquid medium (containing 0.05% cysteine) and cultivated at 37 °C for 18 h, then transferred to fresh MRS liquid medium (containing 0.05% cysteine). ), cultured under the same conditions for 18 hours, centrifuged at 6000g for 15min, washed with 0.9% normal saline and then centrifuged again at 6000g for 10min to obtain the bacteria, resuspended with 20% sucrose solu...

Embodiment 3

[0070] Example 3: Effect of Lactobacillus reuteri on HDM-IgG1 in serum of allergic asthmatic mice

[0071] Specific steps are as follows:

[0072] The 6-week-old SPF grade BALB / c female mice were divided into 5 groups, namely the normal group, the model group and the experimental group. , Lactobacillus reuteri R1 prophylactic group fed with Lactobacillus reuteri R1 and Lactobacillus reuteri R2 prophylactic group fed with Lactobacillus reuteri R2, 6 in each group, raised in the Experimental Animal Center of Jiangnan University, Normal feed, constant temperature 21-26°C, humidity 40-70%, noise less than or equal to 60dB, animal illumination 15-20LX (all animal experiment procedures were reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0073] The experiment lasted for 6 weeks (5 days a week), and the model was established in the 2nd to 6th weeks. The mice in the model group and the experimental group were anesthetized every d...

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PUM

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Abstract

The invention discloses a strain of Lactobacillus reuteri capable of alleviating allergic asthma and an application thereof, belonging to the technical field of microorganisms and the technical field of medicine. Lactobacillus reuteri (Lactobacillus reuteri) of the present invention has the effect of relieving allergic asthma, which is embodied in: (1) significantly reducing the lung inflammatory response of allergic asthma mice; (2) significantly inhibiting the allergic asthma mice serum Dust mite-specific immunoglobulin IgG1 production; (3) significantly reduced the content of IL-5 and IL-13 in the alveolar lavage fluid of allergic asthma mice; (4) significantly reduced the alveolar lavage of allergic asthma mice Therefore, Lactobacillus reuteri (Lactobacillus reuteri) has a huge application prospect in the preparation of products for the prevention and / or treatment of allergic asthma.

Description

technical field [0001] The invention relates to a strain of Lactobacillus reuteri capable of relieving allergic asthma and an application thereof, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Allergic asthma is a chronic airway inflammation involving a variety of cells, especially mast cells, eosinophils and T lymphocytes. In susceptible individuals, this inflammation can cause recurrent episodes of wheezing, shortness of breath, chest tightness, and / or coughing, which can lead to life-threatening complications such as respiratory arrest and respiratory failure. [0003] According to statistics, in the past ten years, there have been about 100 million allergic asthma patients in the world; the prevalence and mortality of allergic asthma in the United States, the United Kingdom, Australia, New Zealand and other countries have gradually increased; there are about 1,000 allergic asthma patients in China. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P11/06A61P37/08C12R1/225
CPCA61P11/06A61P37/08A23L33/135A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2200/314A23V2400/173
Inventor 陈卫陆文伟李凌智翟齐啸张秋香王刚赵建新张灏
Owner 无锡特殊食品与营养健康研究院有限公司
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