Method for identifying wheat scab resistance

Abstract

The invention discloses a method for identifying wheat scab resistance. The method comprises the following steps: inoculating wheat spike base internodes with a gibberella spore liquid after wheat heading, recording the browning condition of an inoculation point, judging whether the inoculation succeeds or not, respectively counting the scab rate on the 7th day, the 14th day and the 21st day afterthe inoculation, and dynamically evaluating the wheat scab resistance. The method has the greatest advantages of fastness and accuracy in observation of the disease condition and identification of the variety resistance, and solves the problems of difficult control of the spore inoculation amount, slow disease incidence, large simultaneous inoculation of the same variety in spike rate difference,high stem corrosion possibility and identification reliability influence of a stem injection method. Compared with the ear base stalk injection method, the method of the invention has the advantagesof consistent and small dosage of the spore liquid at the inoculation part, improvement of the consistency of disease attack, high inoculation efficiency, low cost, obvious phenotypic difference between variety resistance and variety susceptibility, and facilitation of common technicians can judge the gibberella disease resistance of wheat varieties.

Description

technical field [0001] The invention relates to a method for identifying wheat scab resistance, and belongs to the technical field of identification and evaluation of plant disease resistance. Background technique [0002] Stem injection method at the base of the ear is to inoculate the prepared Gibberella spore liquid into the wheat stalk after earing, record the browning situation of the inoculation point, and record the time when the first diseased spikelet appeared. - 25 days to count the rate of diseased spikelets, and evaluate the extended resistance of wheat scab. It is difficult to control the inoculation site of the stem injection method at the base of the ear, and the different inoculation sites affect the susceptibility; the amount of spore liquid is large and difficult to keep consistent, and the pathogenicity of Gibberella is positively correlated with the amount of spores, so the identification of resistance interferes , often lead to inconsistent disease onse...

AI Technical Summary

Problems solved by technology

It is difficult to control the inoculation site of the stem injection method at the base of the ear, and the different inoculation sites affect the susceptibility; the amount of spore liquid is large and difficult to keep consistent, and the pathogenicity of Gibberella is positively correlated with the amount of spores, so the identification of resistance interferes , often lead to inconsistent disease onset time and diseased spikelet rate of the same variety; onset time is slow, and it is difficult to quickly judge the disease; inoculating spore liquid into wheat stems can easily lead to stem rot, and the resistance to wheat scab spikes is extended. Unfavorable identification

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