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Tobacco eIFiso4E-T mutant and application thereof in cultivation of tobacco resisting potato virus Y

A mutant, anti-virus technology, applied in the field of biology, tobacco biotechnology breeding, can solve the problems of unreported germplasm resources, identification of durable anti-PVY resources, disease-resistant breeding and pathogen investigation and identification, etc.

Active Publication Date: 2020-07-10
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Deletion of the tobacco eIF4E1-S gene (abbreviated as eIF4E-1 in some documents, GenBank sequence accession number KF155696) will lead to tobacco resistance to potato virus Y (Potato virus Y, PVY) (Liu Yong et al., 2013; Julio et al., 2014), but there are few studies on resource identification for persistent resistance to PVY, breeding for disease resistance, and investigation and identification of pathogens
There is no report on the germplasm resources of common tobacco persistently resistant to PVY

Method used

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  • Tobacco eIFiso4E-T mutant and application thereof in cultivation of tobacco resisting potato virus Y
  • Tobacco eIFiso4E-T mutant and application thereof in cultivation of tobacco resisting potato virus Y
  • Tobacco eIFiso4E-T mutant and application thereof in cultivation of tobacco resisting potato virus Y

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Embodiment Construction

[0019] The present invention will be further described with reference to the following examples, but the present invention is not restricted in any way. Any changes made based on the teachings of the present invention fall into the protection scope of the present invention.

[0020] Unless otherwise specified, the following examples use conventional methods; unless otherwise specified, the test materials used are all purchased from conventional biochemical reagent companies.

[0021] A tobacco eIFiso4E-T mutant, the amino acid sequence of the tobacco eIFiso4E-T mutant is shown in SEQ ID NO: 2 or SEQ ID NO: 3, which is identical to that of the wild-type tobacco eIFiso4E-T shown in SEQ ID NO:1 Compared with the amino acid sequence, the amino acid sequence of the tobacco eIFiso4E-T mutant was changed from A to T (abbreviation: A76T) at position 76 or from G to D (abbreviation: G98D) at position 98.

[0022] The nucleotide sequence of the wild-type tobacco eIFiso4E-T is shown in SEQ ID N...

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Abstract

The invention discloses a tobacco eIFiso4E-T mutant and an application thereof in cultivation of tobacco resisting potato virus Y. The amino acid sequence of the tobacco eIFiso4E-T mutant is as shownin SEQ ID NO: 2 or SEQ ID NO: 3. Compared with an amino acid sequence of wild tobacco eIFiso4E-T shown as SEQ ID NO: 1, the 76th site of the amino acid sequence of the tobacco eIFiso4E-T mutant is mutated from A to T or the 98th site is mutated from G to D. The eIFiso4E-T mutant is polymerized with a va site resisting the potato Y virus or an eifiso4e-tKO gene, so that the lasting resistance of the tobacco to the PVY can be obtained. The eIFiso4E-T mutant disclosed by the invention has an important significance in cultivating tobacco with lasting resistance to the PVY.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and further belongs to the field of tobacco biotechnology breeding, and specifically relates to the acquisition of tobacco eIFiso4E-T gene mutants and their application in tobacco disease resistance breeding. Background technique [0002] Potato virus Y (PVY) is a typical member of the genus Potyvirus. PVY mainly harms solanaceous crops such as potatoes, tobacco, tomatoes and peppers. In recent years, PVY has risen to become a major disease on tobacco. PVY is non-sexually transmitted by aphids in the field. Due to the short developmental period, strong fecundity and resistance of aphids, the use of chemical agents to control vector insects has limited effectiveness in controlling the disease. Therefore, planting disease-resistant varieties is the most fundamental, most economical and effective means to prevent and control PVY. [0003] Tobacco is an important economic crop and an important model crop for p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H6/82
CPCC07K14/415C12N15/8283
Inventor 刘勇黄昌军李文正于海芹袁诚曾建敏
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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