Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Pregnane Derivatives in the Preparation of Osteoclast Differentiation Inhibitors

A derivative, the technology of pregnane, which is applied in the field of application of pregnane derivatives in the preparation of osteoclast differentiation inhibitors, can solve venous thrombosis, mandibular osteonecrosis, and can not significantly reduce the risk of atypical fractures, etc. question

Active Publication Date: 2021-05-04
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drugs can improve the bone mineral density of patients with osteoporosis to a certain extent, they cannot significantly reduce the risk of atypical fractures, and there are various side effects at the same time, such as bisphosphonates can cause osteonecrosis of the jaw, raloxix Fen can cause venous thrombosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Pregnane Derivatives in the Preparation of Osteoclast Differentiation Inhibitors
  • Application of Pregnane Derivatives in the Preparation of Osteoclast Differentiation Inhibitors
  • Application of Pregnane Derivatives in the Preparation of Osteoclast Differentiation Inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Osteoclast Differentiation Inhibitory Activity Test

[0018]

[0019] 1. Experimental method

[0020] Cell culture: take 8-week-old C57BL / 6 mouse femoral marrow cells, and use MEM-α complete medium containing 10ng / mL M-CSF (containing 10% fetal bovine serum (FBS), 100U / mL penicillin and 100μg / mL streptomycin), cultured in a carbon dioxide incubator at 37°C for 24 hours, discarded the culture medium, washed the cells with phosphate buffered saline (PBS) slowly for 1 or 2 times, removed the suspended cells, and then washed them with 4mL MEM - α complete medium Gently blow off the adherent cells and centrifuge at 1000RPM / min for 10min to obtain mouse bone marrow macrophages (BMMs).

[0021] Induce osteoclast formation: BMMs cells were diluted to a density of 1×10 with MEM-α complete medium containing 10 ng / mL LM-CSF cytokine 5 cells / mL of cell suspension, and seeded in 96-well plate, 100 μL per well. After culturing for 24 hours to adhere to the wall, the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of pregnane derivatives in the preparation of osteoclast differentiation inhibitors. Application of pregnane derivatives in the preparation of osteoclast differentiation inhibitors. The structural formula of the pregnane derivatives is shown in Formula 1. The present invention finds through experiments that the pregnane derivatives have a direct and significant inhibitory effect on the differentiation of osteoclasts, so they can be used as osteoclast differentiation inhibitors to treat various diseases caused by osteoclast differentiation. such as osteoporosis.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of pregnane derivatives in the preparation of osteoclast differentiation inhibitors. Background technique [0002] Osteoporosis is a systemic bone metabolic disease. With the prolongation of human life span and the acceleration of population aging, the incidence of osteoporosis is increasing year by year. It has become one of the important chronic diseases that affect people's quality of life. public health issues. According to statistics at the 2014 American Society for Bone and Mineral Research (ASBMR) annual meeting, osteoporotic fractures are one of the most important chronic diseases in the 21st century, and 1 / 2 of the elderly women and 1 / 3 of the elderly men in the world will suffer from osteoporosis. Loose fractures. Therefore, the development of effective anti-osteoporosis drugs is of great significance for improving the quality of life of humans, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/57A61P19/10
CPCA61K31/57A61P19/10
Inventor 刘志红黄丹娥徐峻谢黎炜顾琼郑双佳许国焕韩木兰
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com