Process for the production of lipidic vehicles
A lipid carrier and liposome technology, applied in the field of producing lipid carriers, can solve the problems of unsatisfactory carrier size and polydispersity index, large amount of resources, complexity and the like
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0056] HSPC: Preparation of stearylamine liposomes
[0057] HSPC (30.0 mg) and stearylamine (0.3 mg) in a molar ratio of 9:0.25 were weighed and placed in a large spherical flask. 3 mL of pure water with dissolved glycerol 20 / 15 / 10% v / v was added to the flask and the mixture was vortexed briefly. A magnet was placed in a silicone oil bath, and the mixture was heated at 60° C. while stirring at 700 rpm for 1 hour. Then, under the same stirring, the suspension was heated at 90° C. for 1 hour. The resulting suspension was cooled to room temperature at a rate of 3 °C / min and a 50 uL sample was extracted which was diluted with 2950 uL of HPLC-grade water and measured by photon correlation spectroscopy (PCS) to calculate the size of the liposomes , polydispersity and zeta-potential. Then, the suspension was heated at 90° C. and 700 rpm for 1 hour, and after cooling, a 50 uL sample was measured again. The results of the prepared liposomes are shown in Table 1, while the size dist...
Embodiment 2
[0068] Preparation of eggPC:cholesterol:stearylamine liposomes
[0069] EggPC (30.0 mg), cholesterol (0.8 / 3.0 mg) and stearylamine (0.3 mg) in a molar ratio of 9:0.5:0.25 or 9:1.8:0.25 were weighed and placed in a large spherical flask. 3 mL of pure water with dissolved glycerol 20% v / v was added to the flask, and the mixture was vortexed for a brief period. A magnet was placed in a silicone oil bath, and the mixture was heated at 60° C. while stirring at 700 rpm for 1 hour. Then, the suspension was heated at 90° C. for 2 hours under the same stirring. The resulting suspension was cooled to room temperature at a rate of 3°C / min, and a 50 uL sample was extracted, diluted with 2950 uL HPLC-grade water and measured with PCS to calculate liposome size and polydispersity. The results of the prepared liposomes are shown in Table 2, while the size distribution of strength, volume and number of particles using 1.8 cholesterol molar ratio is shown in Table 2. figure 2 As shown in ,...
Embodiment 3
[0079] Physical Stability of Liposomes Prepared by the Method of the Invention
[0080] Liposomes containing HSPC:stearylamine 9:0.25, eggPC:cholesterol:stearylamine 9:1.8:0.25 and DSPC:stearylamine 9:0.25 were developed and determined by measuring their size and polydispersity using PCS. Their physical / colloidal stability was assessed for a period of approximately 30 days.
[0081] Weigh HSPC (30.0mg) and stearylamine (0.3mg) of molar ratio 9:0.25, eggPC (30.0mg), cholesterol (3.0mg) and stearylamine (0.3mg) of molar ratio 9:1.8:0.25 and DSPC (30.0 mg) and stearylamine (0.3 mg) in a molar ratio of 9:0.25 and placed in a separate large spherical flask. 3 mL of pure water with dissolved glycerol 20% v / v was added to the flask, and the mixture was vortexed for a brief period of time. A magnet was placed in a silicone oil bath, and the mixture was heated at 60° C. while stirring at 700 rpm for 1 hour. Then, under the same stirring, the suspension was heated at 90° C. for 1 hou...
PUM
| Property | Measurement | Unit |
|---|---|---|
| polydispersity index | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More