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A kind of pathogenic microorganism color developing agent and its preparation method and application

A technology of pathogenic microorganisms and chromogenic reagents, applied in biochemical equipment and methods, measurement/testing of microorganisms, medical science, etc., can solve problems such as the lack of stability for commercial applications, solve incomplete disinfection and reduce infection , the effect of preventing the spread of infectious diseases

Active Publication Date: 2022-07-01
NKD PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2015, the University of Bath reported a method for preparing bacterial chromogenic dressings. In 2018, Donghua University carried out further research on bacterial chromogenic substances based on the research of the University of Bath. Although the preparation process of bacterial chromogenic substances increased Some stabilizers have been added, but the stability is still not up to the standard of commercial application

Method used

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  • A kind of pathogenic microorganism color developing agent and its preparation method and application
  • A kind of pathogenic microorganism color developing agent and its preparation method and application
  • A kind of pathogenic microorganism color developing agent and its preparation method and application

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Embodiment 1

[0031] This embodiment provides a pathogenic microorganism color developing agent of the present invention and a preparation method thereof.

[0032] The specific preparation steps are as follows:

[0033] Prepare 100 mmol / L DPPC (dipalmitate phosphatidyl choline) with 50% ethanol, take 400 μl, add 50 μl 0.2% potassium permanganate solution, reaction temperature -4 °C; ultrasonic vibration, ultrasonic power 800W; reaction time 5h , after the reaction was completed and returned to room temperature, 200 μl (100 mmol / L, prepared with 50% ethanol) cholesterol and 300 μl (100 mmol / L, prepared with 50% ethanol) TCDA were added and mixed, dried by rotary evaporation to form a film, and then 80 μl was added. PBS buffer containing 40mmol / L 5-carboxyfluorescein, fully shaken and mixed, cross-linked by microwave heating, microwave power 500W, cross-linking time 10min; put the cross-linked sample into a container connected to a high-voltage generator device Inside, apply a voltage of 10k...

Embodiment 2

[0035] This embodiment provides a pathogenic microorganism color developing agent of the present invention and a preparation method thereof.

[0036] The specific preparation steps are as follows:

[0037] Prepare 100 mmol / L DPPC (dipalmitate phosphatidyl choline) with 50% ethanol, take 400 μl, add 50 μl of 0.1% perchloric acid solution, the reaction temperature is 0°C; ultrasonic vibration, ultrasonic power 1000W; reaction time 4h, the reaction After returning to room temperature, add 200 μl (100 mmol / L prepared with 50% ethanol) cholesterol and 300 μl (100 mmol / L prepared with 50% ethanol) TCDA to mix, and dry it to form a film by rotary evaporation, and then add 50 μl containing 40 mmol / L. 6-carboxyfluorescein in PBS buffer, fully shaken and mixed, cross-linked by microwave heating, microwave power 900W, cross-linking time 10min; put the cross-linked sample into a container connected to a high-voltage generator device, apply 10kV Voltage, received at a distance of 15cm, and ...

Embodiment 3

[0039] This embodiment provides a pathogenic microorganism color developing agent of the present invention and a preparation method thereof.

[0040] The specific preparation steps are as follows:

[0041] Prepare 100 mmol / L DPPC (dipalmitate phosphatidylcholine) with 50% ethanol, take 400 μl, add 50 μl 0.3% hydrogen peroxide solution, the reaction temperature is 0°C; ultrasonic vibration, ultrasonic power 1000W; reaction time 6h, the reaction After returning to room temperature, add 200 μl (100 mmol / L, prepared with 50% ethanol) cholesterol and 300 μl (100 mmol / L, prepared with 50% ethanol) TCDA to mix, dry it by rotary evaporation to form a film, and then add 80 μl containing 40 mmol / L PBS buffer solution of 5-carboxyfluorescein, fully shaken and mixed, cross-linked by microwave heating, microwave power 500W, cross-linking time 10min; put the cross-linked sample into the container connected to the high-voltage generator device, A voltage of 10 kV was applied, received at a...

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Abstract

The invention relates to the technical field of liposomes, and specifically discloses a pathogenic microorganism color developing agent and a preparation method and application thereof. In the method for preparing a pathogenic microorganism color-developing agent of the present invention, the pathogenic microorganism color-developing agent is carboxyfluorescein liposome, and the preparation raw materials of the carboxyfluorescein liposome include cholesterol, TCDA and oxidized dipalmitic acid Phosphatidylcholine; the molar ratio of the oxidized dipalmitate phosphatidylcholine, the cholesterol and the TCDA is 4:(1.5-2.5):(2-3). The invention effectively improves the storage time of the bacterial color-developing substance, and has high structural stability, simple preparation, small particle size and stable color-developing effect.

Description

technical field [0001] The present invention relates to the technical field of liposomes, in particular to a pathogenic microorganism color developing agent and a preparation method and application thereof. Background technique [0002] In the world where human beings live, there are many microorganisms that are invisible to the naked eye, such as fungi and bacteria. Microbial contamination in public places is common and serious. Investigations and experiments have shown that microorganisms contaminated on environmental surfaces and public goods can not only survive, but also survive for a long time, which provides opportunities for the spread of pathogenic microorganisms. In addition, in the early stage of human wounds, the microbial content is usually maintained at a very low level, and the lower microbial content will not significantly hinder the healing of the wound. This situation generally does not require clinical intervention, but with the proliferation of microorgan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04A61L31/18
CPCC12Q1/04A61L31/18
Inventor 朱翠兰石凌锋陶秀梅
Owner NKD PHARMA CO LTD
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