Method for detecting memory improvement efficacy of health-care food

A memory improvement and health food technology, applied in the direction of testing food, material inspection products, etc., can solve the problems of high cost of feeding mammals, low efficiency of mammalian experiments, long development cycle and so on, and achieves a high number of single egg laying, The effect of memory improvement experimental judgment standard and short test period

Pending Publication Date: 2020-12-04
南京新环检测科技有限公司
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Problems solved by technology

[0003] The above-mentioned prior art has the following defects: (1) the giving time of the test product is generally 27 to 70 days, and the experimental cycle is too long; (3) Due to the high cost of raising mammals and the long development cycle, it is impossible to evaluate multiple test products at the same time, and the efficiency of mammalian experiments Low

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  • Method for detecting memory improvement efficacy of health-care food
  • Method for detecting memory improvement efficacy of health-care food
  • Method for detecting memory improvement efficacy of health-care food

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Embodiment Construction

[0029] The technical solutions of the present invention will be further specifically described below through specific examples.

[0030] In the present invention, unless otherwise specified, all equipment and raw materials can be purchased from the market or commonly used in this industry. The methods in the following examples, unless otherwise specified, are conventional methods in this field.

[0031] The following embodiments of the present invention adopt reagent and instrument to be:

[0032] (1) T maze ( figure 1 ) (customized, Hangzhou Changqing Chemical, China);

[0033] (2) Camera (1200TVL, Sony);

[0034] (3) HD coaxial digital hard disk video recorder (DH-HCVR4104HC-V4, Hangzhou Dahua, China);

[0035] (4) Precision electronic balance (CP214, OHAUS, China);

[0036] (5) Bisphenol AF, CAS number: 1478-61-1, manufacturer: Aladdin

[0037] (6) Edaravone, CAS number: 89-25-8, manufacturer: Aladdin

[0038] Experimental animals:

[0039] Wild-type AB strain zebraf...

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Abstract

The invention relates to the technical field of health-care food inspection and evaluation, in particular to a method for detecting the memory improvement efficacy of health-care food, which comprisesthe following steps of (1) randomly selecting a plurality of zebra fishes, performing memory training, adding an inducer, and establishing a memory disorder model control group, (2) after memory training, putting an inducer and a to-be-tested sample, and establishing a sample group, (3) after memory training, putting an inducer and edaravone, and establishing a positive control group, (4) conducting memory training and establishing a normal control group, and (5) respectively carrying out T-maze experiments on zebra fishes of the memory disorder model control group, the sample group, the positive control group and the normal control group, recording the latency time (T) of the zebra fishes from the starting point to the eutrophication area, and calculating the memory improvement efficacy(%) of the to-be-tested sample. The detection method has the advantages that the test period is short, the judgment standard of a memory improvement experiment is simple, and a plurality of test articles can be simultaneously carried out.

Description

technical field [0001] The invention relates to the technical field of health food inspection and evaluation, in particular to a method for detecting the memory-improving effect of health food. Background technique [0002] With the improvement of people's living standards, more and more people pay attention to health preservation, and health food that enhances memory is more and more popular. In order to ensure the health of consumers, the detection of the memory improvement effect of health food is particularly important. "Technical Specifications for Health Food Inspection and Evaluation (2003 Edition)" pointed out that there are animal experiments and human food test experiments to assist in improving memory function. Animal experiments include platform jumping experiments, dark avoidance methods, shuttle box experiments, and water maze experiments. If any two of the four experiments have positive results, and the repeated positive results are consistent, it can be deter...

Claims

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Application Information

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IPC IPC(8): G01N33/02
CPCG01N33/02
Inventor 俞航萍梁晓晴王钦文朱晓宇徐懿乔李春启
Owner 南京新环检测科技有限公司
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