Moving reaction boundary-based nucleic acid detection method for nucleic acid amplification-chip electrophoresis titration sensing

Abstract

The invention relates to a moving reaction boundary-based nucleic acid detection method for nucleic acid amplification-chip electrophoresis titration sensing. The method comprises the following stepsof designing the nucleic acid amplification-chip electrophoresis titration sensing, carrying out nucleic acid amplification to form double-stranded DNA, carrying out chip electrophoresis titration sensing on the double-stranded DNA, and enabling the double-stranded DNA to enter an electrophoresis channel for a reaction to form the moving reaction boundary; firstly determining interface migration distances of the double-stranded DNA after amplification of a series of nucleic acids with known concentrations, and establishing a standard curve of the interface migration distances and the nucleic acid concentrations; and then carrying out amplification-electrophoresis titration sensing on nucleic acid to be detected to obtain the interface migration distance of the nucleic acid to be detected,and obtaining the concentration of the nucleic acid to be detected according to the established standard curve. The method has the advantages of being quantitative, visible, easy and convenient to operate, high in sensitivity, good in specificity, low in cost and the like.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a nucleic acid detection method based on mobile reaction interface nucleic acid amplification-chip electrophoresis titration sensing. Background technique [0002] As the carrier of biological genetic information, nucleic acid exists widely in all organisms and plays an important role in many biological processes, including cell proliferation, gene expression and apoptosis. Abnormal expression of nucleic acid is closely related to various diseases (such as tumor, viral infection, bacterial infection, metabolic disease). Therefore, nucleic acid detection is of great significance for clinical diagnosis, and nucleic acid detection plays an irreplaceable role in agricultural production, microbial identification analysis, drug development, and environmental analysis. [0003] Numerous methods for nucleic acid bioanalysis have been established. These methods involve Northern blotting...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a nucleic acid amplification-chip electrophoresis titration sensor based on a mobile reaction interface in order to solve the shortcomings of the existing nucleic acid detection methods such as complex operation process, lack of visualization, high cost, and low sensitivity. Detection method

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