Hemoglobin assay reagent, assay kit and assay method
A hemoglobin and measurement method technology, applied in immunoassay, biological test, anti-animal/human immunoglobulin, etc., can solve the problem of lower hemoglobin measurement value
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Embodiment 1-1～4-2
[0085]
[0086] [Preparation of assay reagents]
[0087] As measurement reagents, a first reagent and a second reagent are prepared.
[0088] As the first reagent, 50 mM HEPES buffer (pH 7.4) was used.
[0089] As the second reagent, 50 mM HEPES buffer (pH 7.4), each polystyrene latex loaded with two or more anti-hemoglobin monoclonal antibodies (anti-Hb antibodies), and polystyrene latex loaded with anti-haptoglobin monoclonal antibodies were mixed. Vinyl latex was mixed at the concentrations shown in Table 1 to prepare reagents for measuring immunoagglutination in Examples 1-1 to 1-4 and Comparative Example 1 having the final latex concentrations shown in Table 1. Similarly, the second reagent was mixed at a predetermined concentration to prepare Examples 2-1 to 2-3, Examples 3-1 to 3-3, and Example 4 having the final latex concentrations shown in Table 2. Reagents for measuring immunoagglutination reactions of -1 to 4-2.
[0090] As the anti-haptoglobin monoclonal anti...
Embodiment 5-1～5-5
[0113]
[0114] [Preparation of assay reagents]
[0115] As measurement reagents, a first reagent and a second reagent are prepared.
[0116] As the first reagent, 50 mM HEPES buffer (pH 7.4) was used.
[0117]As the second reagent, 50 mM HEPES buffer (pH 7.4), each polystyrene latex loaded with various anti-hemoglobin monoclonal antibodies (anti-Hb antibodies), and polystyrene loaded with anti-haptoglobin monoclonal antibodies The latexes were mixed at the concentrations shown in Table 6, and the reagents for measuring the immunoagglutination reaction of Examples 5-1 to 5-5 and Comparative Example 8 having the final latex concentrations shown in Table 6 were prepared.
[0118] As anti-haptoglobin monoclonal antibody, AO-53 (α chain recognition) or AN12-8 (β chain recognition) was used. As for the anti-haptoglobin antibody, it was confirmed that free haptoglobin does not aggregate with the insoluble carrier carrying the anti-haptoglobin antibody in the amount added in the ...
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