Breeding method of rapid growth strains of all-male macrobrachium rosenbergii

A technology of Macrobrachium rosenbergii strains, which is applied in the field of breeding of fast-growing strains of all male Macrobrachium rosenbergii, can solve the problems of high seed production cost, reduced growth speed, and smaller growth individuals, and achieves low inbreeding coefficient, The effect of fast growth rate and high male ratio

Active Publication Date: 2021-05-28
ZHEJIANG INST OF FRESH WATER FISHERIES
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Problems solved by technology

Therefore, the culture of all male Macrobrachium rosenbergii has broad market prospects; although Israel has begun to use RNAi technology to interfere with the IAG gene to produce "false female" Macrobrachium rosenbergii and thus produce all-male strains, the production cost of its seedlings is high and limits Moreover, the existing all-male Macrobrachium rosenbergii seed production system does not involve selected components, and the offspring have a high inbreeding coefficient, which is prone to germplasm degradation such as smaller growth individuals, reduced growth speed, and poor stress resistance. Phenomenon

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[0062]The present invention will be specifically described below with reference to the accompanying drawings and specific examples.

[0063]Breeding method of rapid growth line of full-ranted mahogy shrimp, including the following steps:

[0064]Step 1, the establishment of the whole male product;

[0065]The establishment of the full male product includes: Based on the Burmese bungery, combined with RNAi interference DMRT1 gene prepares "false" shrimp, use "false" Roche Maimi and normal male and shrimp, mahogandfront. The full monarchy, establishing a total of the full talentian mahogami. In the future, the male shrimp seedlings will be selected each year, and the siRNA of DMRT1 will be targeted.<60) Transfection, screening of "fake" ♀zzz individuals in which females are female; as subsequent parent, the full-heroic product is mature, strictly independently. Specific content is disclosed in the previous application, and the application number is: 2020101790934; the patent name is: a method ...

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Abstract

The invention discloses a family breeding method of all-male macrobrachium rosenbergii. The method comprises the following steps: establishing an all-male macrobrachium rosenbergii strain by taking Burmese macrobrachium rosenbergii as a basic population and combining a technology for preparing false female shrimps by RNAi (Ribonucleic Acid Interference); on the basis of a macrobrachium rosenbergii NanTaihu No.2 culture population, combining other collection populations to establish a family, and estimating a breeding value of each macrobrachium rosenbergii according to a BLUP method by using ASReml software and sorting the breeding values; carrying out individual breeding, family breeding and intra-family breeding; establishing more than 200 families in each generation; selecting excellent male shrimps and female shrimps as next-generation breeding shrimps, and establishing a second-generation breeding family; and after five years of passage of the family, selecting high-quality male shrimps from the family as male parents every year, and performing mating with false female shrimps to breed, so as to finally obtain rapid-growing all-male strains. The bred all-male strains have excellent characters of parents, inbreeding depression can be avoided, and the obtained all-male macrobrachium rosenbergii is fast in growth, high in quality and suitable for low-density and large-scale breeding.

Description

Technical field[0001]The present invention relates to the field of agricultural aquatic products, in particular a breeding method of rapid growth of full-male larvest.Background technique[0002]Macrobrachiumroenbergii is the long arm shrimp, and the matrix is ​​a variety of freshwater shrimps in the southeast region of my country. Because of its delicious taste, delicate meat, rich nutritious, and fresh listing, it is increasingly being loved and welcomed by our people, and maintains a good market demand and stable sales price. Roche's mahogami male gender character is significant, the male shrimp is large, growing fast, growing up, the market, the southern pops is eating prawn. Therefore, the total market prospect is a broad market prospect; although Israel has begun to use RNAi technology to interfere with the "false" Roche mahogy shrimp and the full-heroic product of the production, the production cost is high and restricted. It has been promoted in the Chinese market; and the exi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59
CPCA01K61/59Y02A40/81
Inventor 徐宾朋陈雪峰高强彭菲程海华
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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