Breeding method for a fast-growing line of all-male Macrobrachium rosenbergii

Abstract

The invention discloses a family selection and breeding method of all-male Macrobrachium rosenbergii. The Burmese Macrobrachium rosenbergii is used as the basic population, and the technique of preparing "pseudo-female" shrimp is combined with RNAi interference to establish a full-male Macrobrachium rosenbergii strain; Based on the breeding group of South Taihu Lake No. 2, combined with other collection groups to form a family, the ASReml software was used to estimate the breeding value of each shrimp according to the BLUP method and ranked; individual selection, family selection and intra-family selection were carried out; each generation A total of more than 200 families have been established; excellent male and female shrimp are selected as the next generation of breeding shrimp, and the second-generation selective breeding family is established; after the family has been passed down for 5 years, high-quality male shrimp are selected from the family as the male parent every year. Pseudo-female "prawns" are bred for mating, and finally a fast-growing all-male line is obtained; the selected all-male line has the excellent traits of both parents, and can also avoid inbreeding decline. Suitable for low-density, large-scale farming.

Description

technical field [0001] The invention relates to the field of agricultural and aquatic products, in particular to a breeding method for a fast-growing line of all-male Macrobrachium rosenbergii. Background technique [0002] Macrobrachium roenbergii is a species of Long-arm shrimp family and macrobrachium genus, and it is an important freshwater shrimp culture species in southeastern my country. Because of its delicious taste, tender meat and rich nutrition, it can be marketed fresh. There are significant differences in the gender representation of males and females of Macrobrachium rosenbergii. The males are large in size, grow fast, and have a growth advantage. In the market, eating prawns is popular in the south. Therefore, all-male M. rosenbergii culture has broad market prospects; although Israel has begun to use RNAi technology to interfere with IAG genes to produce "pseudo-female" M. It is popularized in the Chinese market; and the existing all-male M. rosenbergii se...

AI Technical Summary

Problems solved by technology

Therefore, the culture of all male Macrobrachium rosenbergii has broad market prospects; although Israel has begun to use RNAi technology to interfere with the IAG gene to produce "false female" Macrobrachium rosenbergii and thus produce all-male strains, the production cost of its seedlings is high and limits Moreover, the existing all-male Macrobrachium rosenbergii seed production system does not involve selected components, and the offspring have a high inbreeding coefficient, which is prone to germplasm degradation such as smaller growth individuals, reduced growth speed, and poor stress resistance. Phenomenon

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