A pH-controllable stem cell culture perfusion device
A stem cell culture and perfusion technology, applied in the field of stem cell culture perfusion devices, can solve problems such as stem cell damage, achieve the effects of improving cell culture efficiency and speeding up adaptation as soon as possible
Active Publication Date: 2022-07-26
上海揽微赛尔生物科技有限公司
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Problems solved by technology
[0005] In order to solve the above-mentioned technical problems, the present invention provides a stem cell culture perfusion device with controllable pH value, to solve the problem that the existing stem cell culture perfusion device needs more The stirring mechanism is used for mixing and stirring to speed up the adaptation of the stem cells after freezing and thawing. However, through the setting of the stirring mechanism, its direct contact can easily cause damage to the stem cells, and there are insufficient defects.
Method used
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[0029] as attached figure 1 to the attached Image 6 shown:
[0030]The present invention provides a pH-controllable stem cell culture perfusion device, comprising: a cylindrical casing 1, a cylindrical main body 2 is fixedly installed inside the cylindrical casing 1, and a lifting rod 3 is slidably connected in the cylindrical main body 2 The cylindrical shell 1 includes a perfusion cavity 101, an oblique flow slope 102 and a perfusion output hole 103. The cylindrical shell 1 is provided with a perfusion cavity 101, and the left and right sides of the bottom surface of the inner end of the perfusion cavity 101 are symmetrically provided with oblique flow. The slope 102, the inclined surface of the oblique flow slope 102 and the bottom surface of the inner end of the cylindrical shell 1 form an included angle of 25 degrees, and a perfusion output hole 103 is opened in the center of the bottom surface of the inner end of the perfusion cavity 101; the cylindrical shell 1 includ...
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The invention provides a pH-controllable stem cell culture perfusion device, which relates to the technical field of stem cell culture and perfusion, and solves the problem that after the stem cells are injected into the existing stem cell culture perfusion device, in order to speed up the adaptation of the stem cells after being cold-thawed as soon as possible and improve the cell culture efficiency, The stirring mechanism is often used for mixing and stirring, but the direct contact of the stirring mechanism can easily cause damage to the stem cells, and there is a problem of insufficient defects. A pH-controllable stem cell culture perfusion device comprises a cylindrical shell, a cylindrical main body is fixedly installed inside the cylindrical shell, and a lifting rod is slidably connected in the cylindrical main body. After the stem cells are added into the perfusion cavity from the perfusion input hole, they will first contact the flow baffle and slide down the two flow baffles in turn under their own weight. Mix well to speed up the adaptation of stem cells after thawing and improve cell culture efficiency.
Description
technical field [0001] The invention belongs to the technical field of stem cell culture and perfusion, and more particularly, particularly relates to a pH-controllable stem cell culture and perfusion device. Background technique [0002] Stem cells are a kind of pluripotent cells with self-replication ability. Under certain conditions, they can differentiate into a variety of functional cells. According to the developmental stage of stem cells, they can be divided into embryonic stem cells and adult stem cells. According to the developmental potential of stem cells, they can be divided into three types. Class; totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are under-differentiated and immature cells with the potential to regenerate various tissues and organs and the human body. They are called "universal cells" in the medical community. [0003] For example, application number: CN201811097231.3 The present invention provides a stem cell c...
Claims
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IPC IPC(8): C12N5/00C12M3/00C12M1/36C12M1/34C12M1/12C12M1/04
CPCC12M23/02C12M27/20C12M29/04C12M29/06C12M41/26
Inventor 孔祥玲
Owner 上海揽微赛尔生物科技有限公司