Dual-heart disease animal model construction method based on syndrome of intermingled phlegm and blood stasis and feed composition

Abstract

The invention belongs to the technical field of medicine, and discloses a dual-heart disease animal model construction method, time and feed formula based on the syndrome of intermingled phlegm and blood stasis. A rat high-fat feed formula comprises 2% of cholesterol, 5% of lard, 0.2% of sodium cholate, 20% of cane sugar and 72.8% of basal feed. The method comprises the following steps that: feeding with the rat high-fat feed for 8 weeks is performed; on the first day of modelling, rats receive various unpredictable stresses every day, including nine kinds of stimuli such as fasting for 24 hours, depriving for 24 hours, swimming in ice water, binding for 3 hours, shaking mouse cages, reversing day and night, heating, overnight wet padding and overnight inclination of mouse cages at the temperature of 45 DEG C; the same kind of stimuli discontinuously appear, so that the animals cannot be subjected to unpredictable stimuli; 3 mg / kg of Isoprotenol hydrochloride is subcutaneously injected to the abdomen every day from the sixth week of moulding; and the injection is continued for 3 weeks. The method disclosed by the invention is high in operability, low in animal death rate, easy to evaluate, and low in cost, and is a dual-heart disease animal model with combined diseases and syndromes and intermingled phlegm and blood stasis syndromes.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for constructing an animal model of double-heart disease based on the syndrome of mutual accumulation of phlegm and blood stasis, and a feed composition. Background technique [0002] Coronary atherosclerotic heart disease (CHD) is referred to as coronary heart disease. Coronary heart disease is one of the diseases with the highest fatality rate in the world, and it is currently the first cause of death recognized by the World Health Organization (WHO) [1] . Depression is a mental and emotional disorder with persistent low mood as the main symptom, characterized by high prevalence, high relapse rate, and high suicide rate [2] . According to the WHO report, there are currently 322 million patients with depression worldwide, and the number of patients has increased by 18.4% from 2005 to 2015. [3] , by 2020, depression will be the second leading disease in ...

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Problems solved by technology

[0006] (2) If myocardial ischemia is caused by ligation of the left anterior descending coronary artery in rats, the mortality rate of rats is also very high;

[0007] (3) Due to the widespread existence of people overeating fat, sweet and delicious food, lack of exercise, and many patients with coronary heart disease also have hyperlipidemia, there is currently no literature report on a rat model of myocardial ischemia in a state of hyperlipidemia

[0008] (4) There is currently no report of a composite animal model of coronary heart disease combined with depression

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