Serum tsRNA marker related to liver cancer, probe and application thereof

A marker and serum technology, applied in the field of molecular biology, can solve the problems of serum tsRNA to be discovered, and achieve the effect of improving sensitivity and specificity and easy detection

Pending Publication Date: 2021-11-02
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application prospect of serum tsRNA in the non-invasive diagnosis of liver cancer still needs to be explored. If the abnormally expressed serum tsRNAs in liver cancer can be screened as biomarkers, and corresponding diagnostic kits can be developed, the diagnosis status of liver cancer will be greatly improved. promote

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  • Serum tsRNA marker related to liver cancer, probe and application thereof
  • Serum tsRNA marker related to liver cancer, probe and application thereof
  • Serum tsRNA marker related to liver cancer, probe and application thereof

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Embodiment Construction

[0024] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0025] A serum tsRNA marker associated with liver cancer, wherein the serum tsRNA marker is any one of the following sequences or a combination comprising the sequences:

[0026] tRF-Gln-TTG-006: SEQ ID No.1;

[0027] A probe, which can specifically capture the above-mentioned serum tsRNA markers related to liver cancer. The probe is a custom-synthesized TaqMan tsRNA probe from Applied Biosystems.

[0028] Application of the above-mentioned serum tsRNA markers and probes in the preparation of liver cancer diagnostic kits.

[0029] A diagnostic kit for liver cancer, the kit is used to detect the expression level of the above-mentioned tsRNA markers in serum.

[0030] The above-mentioned probes are included in the kit. The kit also includes commonly used reagents and enzymes for PCR reactions. The kit includes dNTP / AMV reverse transcriptase...

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Abstract

The invention discloses a serum tsRNA marker related to liver cancer and application of the serum tsRNA marker, and belongs to the field of molecular biology. The marker is tRF-Gln-TTG-006 in the blood serum. The tsRNA screened by the invention has relatively high specificity and sensitivity on liver cancer patient detection, and the accuracy of liver cancer diagnosis can be greatly improved. The marker can be used for preparing a diagnostic kit for auxiliary early diagnosis of liver cancer.

Description

[0001] field of invention [0002] The invention belongs to the field of molecular biology, and relates to a serum tsRNA marker related to liver cancer, a probe and an application thereof. Background technique [0003] Hepatocellular carcinoma (HCC) accounts for the highest proportion of primary liver cancer. By 2020, HCC will account for the third in the global tumor-related deaths, and its incidence rate will rank sixth in the world. HCC remains one of the most common cancers in East Asia, including China, with significantly higher incidence, prevalence, and activity than other regions. Liver cancer diagnosed in the early stage can be treated by resection or liver transplantation, and the 5-year survival rate is higher than that of advanced liver cancer. At present, imaging techniques such as CT, ultrasound, and MRI have been applied to the early diagnosis of HCC, but these techniques are difficult to detect lesions with a diameter of ≤1 cm, which is not enough to detect HC...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/686C12N15/113
CPCC12Q1/6886C12Q1/686C12Q1/6869C12Q2600/158C12Q2561/113C12Q2563/107C12Q2545/114C12Q2535/122
Inventor 王延博詹守斌王清颜
Owner NANJING UNIV
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