Reagent strip for quantitative combined detection of follicle-stimulating hormone and luteinizing hormone and its application method

A technology of luteinizing hormone and follicle-stimulating hormone, which is applied in the field of immunoassay and can solve problems such as less research

Active Publication Date: 2021-04-23
石家庄洹众生物科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on FSH, LH and ovarian cancer at home and abroad mainly focuses on the mechanism, but there are few studies on serum FSH, LH and ovarian cancer clinicopathological characteristics, patient prognosis and the value of combined detection of the two in predicting ovarian cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent strip for quantitative combined detection of follicle-stimulating hormone and luteinizing hormone and its application method
  • Reagent strip for quantitative combined detection of follicle-stimulating hormone and luteinizing hormone and its application method
  • Reagent strip for quantitative combined detection of follicle-stimulating hormone and luteinizing hormone and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]This embodiment relates to a reagent strip for the quantitative combined detection of follicle-stimulating hormone and luteinizing hormone and its preparation.

[0032] Reagent strip for quantitative combined detection of follicle stimulating hormone and luteinizing hormone, the structure can be as follows figure 1 As shown, the backing 1, sample pad 8, binding pad 4, NC membrane 2 and absorbent pad 3 are assembled and cut to prepare a double-antibody sandwich colloidal gold immunochromatography reagent strip. The technical core lies in the binding pad and NC film;

[0033] The binding pad 4 is prepared by forming a conjugate of mouse anti-α-FSH monoclonal antibody and colloidal gold label and spraying it on the glass fiber membrane, and the concentration of each milliliter of colloidal gold-labeled mouse anti-α-FSH monoclonal antibody is 30 μg;

[0034] The NC membrane 2 is drawn on the nitrocellulose membrane by mouse anti-β-LH monoclonal antibody, mouse anti-β-FSH mo...

Embodiment 2

[0039] This embodiment relates to the sensitivity verification of the reagent strip of the present invention for blood sample detection and the drawing of a joint detection standard curve.

[0040] Simulate the blood sample added with FSH and LH, the initial concentration is 200mIU / mL, press 2 n (n=0, 1, 2...) Doubling dilution, the concentration after dilution is 200mIU / mL, 100mIU / mL, 50mIU / mL, 25mIU / mL, 12.5mIU / mL, 6.25mIU / mL, and set blank 0mIU / mL, and then carry out the combined determination of FSH and LH.

[0041] Combined measurement operation: use a pipette to take 100 μL of diluted blood sample and blank sample, drop them on 7 sample pads, and then insert it on the colloidal gold analyzer. After 10 minutes, connect the instrument to the computer and read the corresponding OD value.

[0042] According to the test data, draw the relationship diagram between different dilutions of FSH and LH and OD value, as shown in image 3 As shown, and the standard curve diagram of...

Embodiment 3

[0045] This embodiment relates to the verification of the test results of the patient's blood samples using the reagent strip of the present invention.

[0046]

[0047] The optimal cut-off values ​​of serum FSH and LH for diagnosis of serous ovarian cancer patients were 29.12m IU / ml and 26.76m IU / ml, respectively, obtained from blood samples of patients.

[0048] In summary, the reagent strip for the quantitative joint detection of follicle stimulating hormone and luteinizing hormone obtained by adopting the technical scheme of the present invention is obtained by selecting the antibody substance of the colloidal gold-labeled conjugate on the binding pad, and the detection line T1 line on the NC membrane, The antibody substances of the T2 line and the quality control line C line have realized the combined quantitative detection of FSH and LH levels in serum, providing a convenient and accurate detection method for the auxiliary diagnosis of early ovarian cancer.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a reagent strip for the quantitative joint detection of follicle stimulating hormone and luteinizing hormone, which comprises a combination pad and an NC film, and the combination pad is formed by a mouse anti-α-FSH monoclonal antibody and a colloidal gold label and sprayed on it Prepared on a glass fiber membrane; the NC membrane is drawn on the nitrocellulose membrane by mouse anti-β-LH monoclonal antibody, mouse anti-β-FSH monoclonal antibody and anti-mouse IgG polyclonal antibody respectively, and sequentially forms a detection line T1 line, test line T2 line and quality control line C line are prepared. By adopting the reagent strip of the present invention, the combined quantitative detection of follicle-stimulating hormone and luteinizing hormone in serum is realized, which not only provides a convenient and accurate detection method, but also provides a basis for finding highly sensitive and specific tumor markers and serum reference The value range provides a theoretical basis for the auxiliary diagnosis of early ovarian cancer, and has significant application significance for the diagnosis of ovarian cancer.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a reagent strip for the quantitative combined detection of follicle-stimulating hormone and luteinizing hormone, and a method for using the reagent strip. Background technique [0002] Ovarian cancer is currently the gynecological malignancy with the highest mortality rate, among which serous tumors are the most common. According to statistics, 70% of serous ovarian cancer patients are already at an advanced stage when they are diagnosed. Therefore, it is necessary to improve the early diagnosis rate of ovarian cancer patients. Currently, There are many deficiencies in the early diagnosis techniques of ovarian cancer. For example, when ovarian cancer does not metastasize, that is, it is limited to the ovary, ultrasound and other imaging methods are difficult to diagnose; the current diagnosis of ovarian cancer mainly depends on pathological biopsy, but biopsy exists. Invasive...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/76G01N33/577
CPCG01N33/577G01N33/76
Inventor 李晓春齐颖颖
Owner 石家庄洹众生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products