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Application of SLC in preparation of product for preventing testis function decline caused by testis injury

A testis and function technology, applied in the field of medicine, can solve problems such as scarcity, achieve the effects of weakening inflammation, preventing testicular function decline, and restoring fertility

Pending Publication Date: 2022-01-04
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, products that can be used to prevent testicular function decline caused by testicular injury are still very scarce

Method used

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  • Application of SLC in preparation of product for preventing testis function decline caused by testis injury
  • Application of SLC in preparation of product for preventing testis function decline caused by testis injury
  • Application of SLC in preparation of product for preventing testis function decline caused by testis injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Injury of contralateral testicular cells

[0031] 1. Experimental method

[0032] On day 1 when the SLC was transplanted to the rotated side, the contralateral testicular tissues of each group were taken and cut into pieces with micro scissors (the size of the tissue block was 1 mm) 3 ), adding 1 mg / mL type IV collagenase and digesting in a water bath for 15 minutes. The water bath was carried out in a water bath at 37 ° C, and then put into PBS containing 10% BSA to terminate the digestion, and then filtered with a 50 μm filter, and the Centrifuge at 1200 rpm for 3 minutes at 4°C, and resuspend the obtained cell pellet with 300 μL of PBS to obtain a mouse testis cell suspension. Take 50,000 to 100,000 cells, centrifuge to discard the supernatant, add PI and AnnexinV-APC staining liquid, and stain in the dark for 10 minutes before detecting with a flow cytometer.

[0033] 2. Experimental results

[0034] The above stream analysis results and their correspon...

Embodiment 2

[0035] Embodiment 2 The inflammatory reaction of contralateral testis

[0036] 1. Inflammatory cells in the contralateral testis

[0037] On day 1 when the SLC was transplanted to the injured side, the testicular tissues on both sides were taken and cut into pieces with micro scissors (the size of the tissue block was 1 mm) 3 ), adding 1 mg / mL type IV collagenase and digesting in a water bath for 15 minutes. The water bath was carried out in a water bath at 37 ° C, and then put into PBS containing 10% BSA to terminate the digestion, and then filtered with a 50 μm filter, and the Centrifuge at 1200 rpm for 3 minutes at 4°C, resuspend the obtained cell pellet with 300 μL of PBS, and obtain mouse testis cell suspensions on both sides. Take the mouse testis cell suspension on both sides and incubate with IgG respectively, then use CD11b and Ly6G to label testicular neutrophils, use CD11b and Ly6C to label testicular monocytes, and use F4 / 80 and CD45 to label testicular macrophage...

Embodiment 3

[0042] The spermatogenic function of embodiment 3 contralateral testis

[0043] 1. Experimental method

[0044] On day 28 when SLC was transplanted to the injured side, the contralateral testicular tissues of each group were paraffin-sectioned, dewaxed, rehydrated, penetrated with 0.1% TritonX-100 for 10 minutes, blocked with 5% BSA for 1 hour, and then treated with primary antibody After incubation of SYCP3, PNA and α-SMA at 4°C overnight, the sections were washed 3 times with PBS (5 minutes each), and then incubated with Alexa Fluor-488 and AlexaFluor-594 for 1 hour in the dark, and the sections were washed with PBS Wash 3 times (5 minutes each time). After counterstaining the nuclei with DAPI for 10 minutes, wash with PBS and mount the slides.

[0045] 2. Experimental results

[0046] The sealed sections were observed under a confocal microscope, and the results of immunofluorescence staining and their statistics were as follows: Figure 5 As shown, among them, Figure...

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Abstract

The invention provides application of SLC in preparation of a product for preventing testis function decline caused by testis injury. Experiments show that after testis are injured, the SLC can effectively relieve testis cell injury, weaken the inflammatory response of testis, protect the spermatogenic function of testis and recover the fertility of testis; and more importantly, after the unilateral testis is injured, the SLC can effectively relieve injury to the cells of the contralateral testis, weaken inflammatory response of the contralateral testis, protect the spermatogenic function of the contralateral testis and recover the fertility of the contralateral testis, which indicates that the SLC can effectively prevent testis function decline caused by testis injury, and the SLC has good application value in the aspect of developing related products capable of preventing testis function decline caused by testis injury.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it relates to the application of SLC in the preparation of products for preventing testicular function decline caused by testicular damage. Background technique [0002] Testicular damage will cause a series of physiological changes, resulting in decreased testicular function, and even affect fertility. Testicular injuries generally occur in young adults, and patients often experience injuries to the testis, sheath, spermatic cord, and scrotum wall at the same time. The common causes of injury are mostly direct violence, such as injuries caused by bullets, kicking, falling or riding. contusion, etc. [0003] The decline of testicular function caused by testicular damage or even infertility is relatively common. Therefore, looking for products that can prevent the decline of testicular function caused by testicular damage has become one of the current research hotspots. For e...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P15/08A23L33/10
CPCA61K35/28A61P15/08A23L33/10A23V2002/00A23V2200/30
Inventor 迟阿妮张敏邓春华刘汉超杨璧铖
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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