Experimental method for screening FASN inhibitor in peony seed meal monomeric compound based on computer simulation

A technology of peony seed meal and experimental method, which is applied in the field of pharmaceutical analysis, can solve the problems of breast cancer prevention, treatment and prognosis, unsatisfactory effectiveness, and insufficient understanding of the pathogenesis, so as to ensure work efficiency and high safety. Authenticity, the effect of increasing the success rate

Pending Publication Date: 2022-03-01
INST OF BOTANY CHINESE ACAD OF SCI +1
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Problems solved by technology

[0002] Breast cancer is a common malignant tumor that endangers women's health today. Scientists have been committed to finding ways to eradicate this disease. In recent years, although great progress has been made, the survival rate of breast cancer patients has been greatly improved, but its internal pathogenesis It is ...

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  • Experimental method for screening FASN inhibitor in peony seed meal monomeric compound based on computer simulation
  • Experimental method for screening FASN inhibitor in peony seed meal monomeric compound based on computer simulation
  • Experimental method for screening FASN inhibitor in peony seed meal monomeric compound based on computer simulation

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Embodiment Construction

[0081] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0082] Such as Figure 1 to Figure 14 As shown, the present invention provides an experimental method for screening FASN inhibitors in monomeric compounds of peony seed meal based on computer simulation, and the specific operation steps of the experimental method for screening FASN inhibitors in monomeric compounds of peony seed meal by computer simulation are as follows:

[0083] S1, molecular docking;

[0084] S2. Cell viability detection-MTT method;

[00...

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Abstract

The invention belongs to the technical field of pharmaceutical analysis, and discloses an experimental method for screening a fatty acid synthase (FASN) inhibitor in a peony seed meal monomeric compound based on computer simulation, and the experimental method for screening the FASN inhibitor in the peony seed meal monomeric compound based on computer simulation comprises the following specific operation steps: S1, molecular docking; s2, cell activity detection; s3, performing protein immunoblotting; and S4, cell apoptosis detection. Whether four monomeric compounds in the peony seed meal extract have anti-tumor activity caused by inhibition of FASN activity and expression or not is screened by means of a molecular docking method, a homologous modeling means is tried to construct a whole enzyme dimer structure of FASN, and a new thought and a new method are provided for screening of the FASN inhibitor. A large number of invalid experiments can be effectively avoided, the success rate of experiment operation is improved, and the experiment operation cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical analysis, in particular to an experimental method for screening FASN inhibitors in peony seed meal monomer compounds based on computer simulation. Background technique [0002] Breast cancer is a common malignant tumor that endangers women's health today. Scientists have been committed to finding ways to eradicate this disease. In recent years, although great progress has been made, the survival rate of breast cancer patients has been greatly improved, but its internal pathogenesis It is still not fully understood, and the method and effectiveness of breast cancer prevention, treatment and prognosis are still not ideal. Since the 1990s, the expression and activity of FASN found in tissue samples such as breast cancer were much higher than normal levels, making it begin to as potential cancer therapeutic targets. [0003] Although many FASN inhibitors have been reported in the past 20 years...

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Application Information

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IPC IPC(8): C12Q1/02G01N33/545G16B15/30
CPCG01N33/5017G01N33/545G16B15/30C12N2503/02G01N2500/10
Inventor 舒庆艳马晓丰方令豪刘政安邢旭彭丽平王纯妍李旸
Owner INST OF BOTANY CHINESE ACAD OF SCI
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