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Microorganism electrochemical analysis device and analysis method thereof

A technology of microbial electrochemistry and analysis device, applied in the field of microbial electrochemical analysis device, can solve the problems such as inability to accurately obtain microbial electrode reaction test results, lack of electrochemical analysis test methods, complex interference of microbial electrode test results, etc., and achieve accurate solutions. Determination, Reduction of Interfering Factors, Resolving Effects of Accurate Characterization and Efficient Analysis

Pending Publication Date: 2022-07-29
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the non-standardization of experimental conditions, the test results of microbial electrodes contain complex interference, and it is impossible to accurately obtain the test results of the microbial electrode reaction itself
At the same time, there is a lack of electrochemical analysis and testing methods specifically for microbial electrodes. Traditional cyclic voltammetry, linear sweep voltammetry, and methods for measuring polarization curves with resistance boxes have certain limitations.

Method used

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  • Microorganism electrochemical analysis device and analysis method thereof
  • Microorganism electrochemical analysis device and analysis method thereof
  • Microorganism electrochemical analysis device and analysis method thereof

Examples

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Embodiment 1

[0050] A microbial electrochemical analysis device, the structure of which is as follows figure 1 As shown, the device includes a sealed electrolytic cell cavity, a working electrode, a reference electrode, a counter electrode, and an air cathode; wherein the air cathode is arranged on one side of the electrolytic cell cavity, and the reference electrode, the working electrode and the counter electrode are in turn It is arranged on the top of the electrolytic cell cavity (sealed and connected by a hollow nut, which belongs to the detachable type), and the position of the reference electrode is close to the side of the electrolytic cell cavity provided with the air cathode, so that the reference electrode is located between the working electrode and the counter electrode. outside the current loop, so that the electrode potential of the working electrode does not contain solution resistance interference.

[0051] The working electrode is a microbial electrode, the reference elec...

Embodiment 2

[0055] The microbial electrochemical analysis device in Example 1 was used for microbial analysis, and the microorganism to be tested was used as the working electrode in the microbial electrochemical analysis device in Example 1, and the specific method was as follows:

[0056] (1) The microbial electrochemical analysis device in Example 1 is placed in a container containing water (the water in the container can completely submerge the air cathode), and the test is carried out under stirring at a rotating speed of 200 to 500 rpm. The working electrode is connected with the working electrode clip of the electrochemical workstation, the reference electrode in the device is connected with the reference electrode clip of the electrochemical workstation, and the counter electrode in the device is connected with the counter electrode of the electrochemical workstation;

[0057] (2) Control the current and voltage through the electrochemical workstation, and record the data. The spec...

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Abstract

The invention relates to a microbial electrochemical analysis device and an analysis method thereof, and belongs to the technical field of microbial electrochemical analysis. The invention discloses a microbial electrochemical analysis device, which is characterized in that an air cathode is arranged on one side of an electrolytic tank cavity, meanwhile, the device also comprises a counter electrode and a reference electrode, the device can be freely switched between a microbial electrode three-electrode analysis mode and a two-electrode maintenance mode, and a microbial electrode signal is accurately measured through the three-electrode mode; the long-term maintenance requirement of the microbial electrode is met through a two-electrode mode, and the defect that a traditional microbial battery structure cannot meet two requirements at the same time is overcome. The invention also discloses a microbial electrochemical test analysis method, which is characterized in that the microbial electrochemical analysis device disclosed by the invention is connected with an electrochemical workstation, so that the steady-state polarization curve of the microbial electrode and the resistance distribution analysis of the microbial electrode are realized; the problems of accurate characterization and efficient analysis of the performance of the microbial electrode are solved.

Description

technical field [0001] The invention belongs to the technical field of microbial electrochemical analysis, and relates to a microbial electrochemical analysis device and an analysis method thereof. Background technique [0002] Microbial electrodes are mainly used as anodes in electrochemical systems such as fuel cells, sensors, and sewage treatment. At present, the electrochemical performance test experiments of microbial electrodes are mainly carried out in actual complex reaction cells, including the ohmic resistance of electrode materials and solutions, the membrane resistance of cathode and anode separators, the material electric double layer and the counter electrode and other complex factors. Due to the non-standardization of experimental conditions, the test results of microbial electrodes contain complex interference, and the test results of the microbial electrode reaction itself cannot be accurately obtained. At the same time, there is a lack of electrochemical a...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 殷逢俊刘鸿赵迎徐泽宇
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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