Strain of streptomycete for preventing sheath blight of rice

Abstract

The invention discloses a screening technology using Streptomyces, which can obtain Streptomyces strains with strong antagonistic effects on rice sheath blight pathogens and rice blast pathogens through preliminary screening with PAD and YPTG plates, and the strains are ball Streptomyces globisporus H50, which was isolated and screened from the soil in the ecological region of Sichuan Province, my country, was preserved in the China Center for Type Culture Collection on May 2, 2003, and the preservation number is CCTCC NO: M 203034. The invention can antagonize the pathogenic bacteria of rice sheath blight and rice blast, especially has a strong antagonistic effect on the fusion mycelia group AG-1 of Rhizoctonia solani and the seriously harmful ZB15 rice blast physiological race, and its control effect Well, the antibacterial rate reaches more than 65%, effectively solving the two major diseases and insect pests that harm rice.

Description

Technical field: [0001] The present invention relates to a bacterial strain of the genus Streptomyces, in particular to a screening technique utilizing Streptomyces, through the primary screening of PAD and YPTG plate application point connection method, to obtain the pathogenic bacteria of rice sheath blight and rice blast that have strong antagonism Acting Streptomyces strains. Background technique: [0002] Rice sheath blight (Thanatephoms cucumeris) and rice blast (Pyricularia) are two major diseases in rice fields in my country. Since 1987, the occurrence of rice sheath blight and rice blast has become serious every year due to the high temperature and high humidity in the rice fields of Sichuan. Due to the lack of high sheath blight resistance genes in the currently promoted rice varieties, and the use of disease-resistant varieties has not yet achieved satisfactory disease control effects. In addition, with the large-scale promotion of dwarf varieties and hybrid rice...

AI Technical Summary

Problems solved by technology

Due to the lack of high sheath blight resistance genes in the currently promoted rice varieties, and the use of disease-resistant varieties has not yet achieved satisfactory disease control effects. In addition, with the large-scale promotion of dwarf varieties and hybrid rice and the impact of rice field management measures , so the occurrence area of ​​rice sheath blight is expanding year by year, resulting in a loss rate of more than 10% of my country's annual rice output

At present, in actual production, Jinggangmycin is mainly used to prevent and control the disease. Although its effect is good, it has caused the sheath blight bacteria to develop drug resistance due to its singleness and long-term use.

In the Sichuan rice area, rice blast is the most harmful in the physiological race ZB15, but there is still a long way to clone the blast resistance gene from rice plants. Therefore, the genetic engineering of rice blast resistant rice limited, and general chemical pesticides will cause great harm to human health and the environment

As we all know, Streptomyces fungi are important fungi that produce small molecule antibiotic substances, but there are no related reports on the research and utilization of Streptomyces coccidioides in the use of microorganisms to prevent and control crop diseases and insect pests

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