Bamboo forest biological fertilizer

A bio-fertilizer and bamboo forest technology, applied in the direction of organic fertilizers, fertilization devices, fertilizer mixtures, etc., can solve the problems of biological nitrogen fixation that have not been reported, and achieve the goal of improving soil water and fertilizer retention, promoting root growth, and increasing nitrogen content Effect

Inactive Publication Date: 2004-02-18
吴晓丽 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its special growth advantages and important economic value, it has attracted more and more attention from all over the world, but there is no report on its biological nitrogen fixation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1) prepare nitrogen-fixing bacteria test tube solid slant medium, its formula is: glucose 0.5%, sucrose 0.5%, KH 2 PO 4 0.04%, K 2 HPO 4 .3H 2 O 0.01%, NH 4 (SO 4 ) 2 0.1%, MgSO 4 .7H 2 O 0.02%, NaCl 0.01%, CaCl 2 .2H 2 O 0.002%, FeCl 3 .3H 2 O 0.001%, Na 2 MoO 4 .2H 2 O 0.0005%, K 2 SO 4 0.15%, yeast extract 0.06%, agar 2%, pH6.8-7.2. After sterilization, respectively inoculate WG-7 and WG-8 strains, and culture at 30-32°C to make the surface grow full.

[0067] 2) prepare the shaker culture medium of nitrogen-fixing bacteria, its formula is: glucose 0.5%, sucrose 0.5%, corn flour 1%, K 2 SO 4 0.15%, KH 2 PO 4 0.04%, K 2 HPO 4 .3H 2 O 0.01%, NH 4 (SO 4 ) 2 0.1%, MgSO 4 .7H 2 O 0.02%, NaCl 0.01%, CaCl 2 .2H 2 O 0.002%, FeCl 3 .3H 2 O 0.001%, Na 2 MoO 4 .2H 2 O Mix two kinds of liquid bio-fertilizers according to the required volume ratio to become a mixed strain liquid bio-fertilizer.

[0068] 4-2) Put the shaker liquid culture...

Embodiment 2

[0073] In Example 1, two strains of combined nitrogen-fixing bacteria isolated from the rhizosphere of bamboo plants were taken as an example to carry out the production of bamboo forest liquid bio-fertilizer. Also can only use any one strain bacterium in 2 strain bacteriums in actual production, carry out the production of bamboo forest liquid bio-fertilizer with above-mentioned same method.

Embodiment 3

[0075] The peat was crushed, passed through an 80-mesh sieve, dried and sterilized with a hot air drum at 200°C, and cooled to room temperature. Various liquid bio-fertilizers (comprising single strains and mixed strains) made with the method of Example 1 are adsorbed with peat respectively, so that the number of live bacteria of its functional bacteria reaches the required standard, and the water content is less than 30% ( If the water content is too high, low-temperature air drying can be used to make various solid bacterial agents containing single or mixed strains.

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PUM

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Abstract

A biological fertilizer for bamboo forest is prepared from Paenibacillus polymyxa WG-7 and/or Bacillus licheniformis WG-8, and matrix.

Description

technical field [0001] The invention relates to fertilizers containing microorganisms, in particular to biological fertilizers for bamboo forests. Background technique [0002] Chemical fertilizers are easy to use, fast in fertilizer efficiency, and can provide essential elements for crop growth, playing a key role in solving the problem of food and clothing for human beings. However, chemical fertilizers also have many disadvantages, such as: a large amount of energy is consumed during production, and the production cost is high; water and air pollution will be generated during production and use, the loss rate is high, and the utilization rate of plants is low; nitrogen fertilizers accumulate in plants after excessive application of nitrogen fertilizers. Nitrite is harmful to human health; due to the single element of chemical fertilizers, it is difficult to match them in a reasonable ratio, which reduces the nutritional value and flavor of agricultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00C05F11/08C05G3/00C12N1/20
Inventor 吴晓丽顾小平
Owner 吴晓丽
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